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GENETIC TRANSFORMATION AND TRANSIENT EXPRESSION OF -GLUCURONIDASE (GUS) IN SUNFLOWER (Helianthus annuus L.)

KrishiKosh

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Title GENETIC TRANSFORMATION AND TRANSIENT EXPRESSION OF -GLUCURONIDASE (GUS) IN SUNFLOWER (Helianthus annuus L.)
 
Creator ANITHA, V
 
Contributor FARZANA JABEEN
 
Subject GENETIC, TRANSFORMATION, TRANSIENT, EXPRESSION, GLUCURONIDASE, SUNFLOWER
 
Description In the present investigation, tissue culture studies were carried out to
determine the effect of different hormonal concentrations and combinations
on callus induction and growth, to determine the response of explants
(hypocotyl, cotyledonary leaf and primary leaf) for callus induction and
growth in five sunflower genotypes viz., APSH-11, NDSH-1, DRSF-108, BRISF-73
and EC-68415. Studies were conducted to determine the transformation frequency
(in the form of transient GUS expression per cent) in direct explants and their
respective calli of the five sunflower genotypes using particle inflow gun. Between
the hormonal combinations (kinetin + 2,4-D and BA + NAA), MS medium
supplemented with BA + NAA was found better for callus induction from
cotyledonary leaf than MS medium supplemented with kinetin + 2,4-D. Among the
kinetin + 2,4-D treatments, 1.0 mg/l, 2,4-D + 1.0 mg/l kinetin and among BA +
NAA treatments 1.0 mg/l NAA + 1.0 mg/l BA were found better for callus
induction. Among the BA treatments MS medium supplemented with 0.5 mg/l BA
was found better for inducing maximum caulogenesis and rhizogenesis from calli
irrespective of genotype. Among the five sunflower cultivars NDSH-1 was found
better both for callus induction and rhizogenesis, DRSF-108 was better for
caulogenesis.
Transformation frequency in terms of transient GUS expression percentage
was determined in direct explants and their respective calli of five sunflower
genotypes using particle inflow gun. Hypocotyls were found better as target tissue
for particle bombardment among the direct explants as they gave highest transient
GUS expression percentage. Callus showed higher transient GUS expression
percentage than direct explants. The study suggests better transformability of callus
derived by explants of the sunflower genotypes using particle bombardment as the
method of gene transfer.
 
Date 2016-08-16T14:31:02Z
2016-08-16T14:31:02Z
2006
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72608
 
Language en
 
Relation D7840;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD