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Identification of sterility mosaic disease resistant recombinant inbred lines in high yielding background and localizing genomic regions controlling its resistance in pigeonpea [Cajanus cajan (L.) Millsp.]

KrishiKosh

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Title Identification of sterility mosaic disease resistant recombinant inbred lines in high yielding background and localizing genomic regions controlling its resistance in pigeonpea [Cajanus cajan (L.) Millsp.]
 
Creator NEETHA, JAYARAM
 
Contributor RAMESH, S
 
Description Sterility mosaic disease (SMD) of pigeonpea [Cajanus cajan (L.) Millsp.] caused by
pigeonpea sterility mosaic virus (PPSMV) is one of the most important diseases of
pigeonpea which can cause yield loss upto 95 percent under favourable conditions.
Development and deployment of cultivars resistant to SMD is considered as most
economical and eco-friendly option to mitigate the losses due to SMD. Identification of
easily assayable, crop-stage non-specific and environment-neutral surrogates such as
DNA markers linked to genomic regions controlling SMD is a step towards this. In the
present study, 120 RILs derived from SMD susceptible (TTB 7) and resistant parents
(ICP 7035), their parents (TTB 7 and ICP 7035) and two checks (BRG 1 and BRG 2)
were screened for response to SMD following Leaf Stapling technique for use in locating
genomic regions controlling SMD resistance at All India Co-ordinated Research Project
(AICRP) on pigeonpea, University of Agricultural Sciences (UAS), Gandhi Krishi
Vignana Kendra (GKVK), Bengaluru during 2011. The RILs were phenotyped for
economic traits at the experimental plots of the Department of Genetics and Plant
Breeding, UAS, GKVK, Bengaluru during 2011. The RILs 14, 92 and 107 were immune
to the disease (0.00% SMD incidence) and transgressed their resistant parent, ICP 7035
(06.67% SMD incidence). The RILs 03 (10.00% SMD incidence) and 32 (06.67% SMD
incidence) were found resistant to SMD infection with pod and seed yield potential better
than the better parent (TTB 7).The RIL No. 51, 12, 40, 04, 28, 59, 03, 62 and 63
transgressed their parents and BRG 1 and BRG 2 for pod yield potential. One
(ICPMIE04) of the eleven SSR markers polymorphic to parents of RILs could
differentiate SMD resistant and susceptible bulks and their constituent individuals of
RILs. However, single marker analysis indicated lack of evidence for its association with
genomic regions conferring SMD resistance.
 
Date 2016-12-06T12:47:34Z
2016-12-06T12:47:34Z
2012-07-30
 
Type Thesis
 
Identifier Th-10241
http://krishikosh.egranth.ac.in/handle/1/89271
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore