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Development of multiple gene construct with regulatory genes and their functional validation

KrishiKosh

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Title Development of multiple gene construct with regulatory genes and their functional validation
 
Creator K. C, Babitha
 
Contributor M, Udayakumar
 
Subject meat, maize, rice, developmental stages, group communication, economics, planting, livestock, crops, land resources
 
Description Rice is widely cultivated under irrigated condition and its water requirement is very
high. Due to dwindling water resources, to save irrigation water growing rice under
semi-irrigated aerobic condition has phenomenal relevance. In this ecosystem decreased
water availability, high VPD affects crop growth and productivity. From this context it is
important to improve adaptation of rice under aerobic condition by improving water
relations and cellular level tolerance mechanisms. Stress adaptation at cellular level
involves activation of stress responsive genes which are regulated by transcription
factors. Hence, under stress, co-ordinated expression of multiple transcription factors is
crucial to increase cellular level tolerance. As a first step a multigene construct
coexpressing GUS, AtbHLH17 and AtWRKY28 was developed using modified multisite
gateway technology and developed transgenics in Arabidopsis. These transgenics
showed improved tolerance to diverse stresses like drought, salinity and oxidative stress
by upregulation of large number of downstream genes. Further, three stress responsive
TFs from different families i.e EcNAC1, EcMYC57, EcbZIP60 were cloned from
fingermillet and validated their relevance in stress tolerance in tobacco. Tobacco
transgenics co-expressing all the three TFs showed superior phenotype under stress
compared to single TFs expressing transgenics signifying the importance of co
expression of relevant stress specific TFs. To improve adaptation of rice under semiirrigated
aerobic conditions rice transgenics were developed co-expressing EcNAC1,
EcMYC57 and EcbZIP60 in the background of genotype having superior water relations.
Desirable transformants were identified based on dessication response. Molecular
characterization of transformants showed the integration and expression of all three
genes. The transgenics showed improved tolerance to salinity and oxidative stress.
Under drought stress, many promising transgenic lines showed reduced spikelet sterility
and higher yield compared to wild type. The study provides proof of concept that coexpression
of few TFs improved cellular level tolerance and transgenics is a potential
option to combine the relevant traits and improve field level tolerance.
 
Date 2016-05-19T10:32:35Z
2016-05-19T10:32:35Z
2012-11-17
 
Type Thesis
 
Identifier Th-10420
http://krishikosh.egranth.ac.in/handle/1/66105
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru