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Fine Mapping of Candidate Gene for Root Length for Adaptation to Drought, Inheritance and Validation of Gene Based Markers Linked to Aroma in Upland Rice (Oryza sativa L.)
KrishiKosh
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| Title |
Fine Mapping of Candidate Gene for Root Length for Adaptation to Drought, Inheritance and Validation of Gene Based Markers Linked to Aroma in Upland Rice (Oryza sativa L.)
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| Creator |
Prakash Irappa Gangashetti
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| Contributor |
P.M. Salimath
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| Subject |
Genetics & Plant Breeding
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| Description |
An investigation was carried out to fine map the candidate gene for root length qAZRL9 on chromosome 9 and its validation in local land races of rice; morphological and molecular characterization of local short grain aromatic genotypes, inheritance and validation of gene based markers linked to aroma. The qAZRL9 (RM242-RM201) was fine mapped to small genomic regions between RM24569-RM242 (0.13 Mbp) and RM5661-RM24579 (0.40 Mbp). The marker RM242 was found to be closely linked to increased root length in local land races of rice, thus can be effectively used in MAS and MABC programmes. Morphological characterization of 42 landraces revealed that, landrace Gandhasali was superior for test weight and grain yield per plant, Pusa suganda-4 was superior for grain yield per plant while, Kalanamak and Ambemohor were rich in Fe and Zn in grains. Genetic diversity analysis indicated that inter cluster distance was not consistent with the geographic distribution of land races. The land races belonging to diverse ecological regions were clustered together, whereas, land races of the same region were grouped into separate clusters. Grain yield per plant had maximum contribution towards the genetic divergence followed by grain length and plant height. Molecular characterization by SSR and InDel markers revealed presence of variation between basmati and short grain aromatic genotypes. Most of the basmati genotypes were grouped to one cluster while, all the local short grain aromatic genotypes were grouped into another cluster. These markers can be used for preventing adulteration of Indian basmati rice with other basmati rice. Study on inheritance pattern of aroma in three populations viz., the F2 of IR-64 × Ambemohor, F7 RILs of IR-38 × Jeerige sanna and BC2F2 of Kalinga III × Azucena NILs indicated that, aroma was controlled by single recessive gene. The validation of gene based markers linked to aroma has shown that BAD II had maximum phenotypic variation; hence can be used for MAS programmes for selecting aromatic genotypes in segregating populations. |
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| Date |
2016-07-25T14:21:40Z
2016-07-25T14:21:40Z 2012 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/69798
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| Format |
application/pdf
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| Publisher |
UAS Dharwad
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