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Genetic divergence analysis in date palm (Phoenix dactylifera L.) using ISSR markers

KrishiKosh

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Title Genetic divergence analysis in date palm (Phoenix dactylifera L.) using ISSR markers
 
Creator Deepak
 
Contributor Dhillon, Santosh
 
Subject Date palm, ISSR, Genetic diversity
 
Description Date palm (Phoenix dactylifera L.) is a dioecious,
monocotyledonous woody perennial belonging to the family Palmae
(Arecaceae) and grown in the arid regions of the world for its nutritious
fruit. It has the distinction of being one of the oldest fruit tree of the
world. This study was thus undertaken to assess polymorphism among
ten male and thirty female genotypes of eight varieties of date palm viz.
Zaglool, Egypt No. 2, Hillawi, Zaidi, Sayer, Khadrawy, Shamran and
Medjool using 40 ISSR primers. A total of 160 amplified products were observed of which 8 were monomorphic and 152 were polymorphic.
Average polymorphism across the 30 genotypes was 89.46±6.8 %. The
number of amplified DNA bands varied from 2 to 13 with an average of
7.27±0.77 bands per primer. One ISSR primer (BH-08) amplified a
unique allele of 630 bp which was present in all male plants but was
absent in all female plants included in the study, suggesting it to be a
useful marker for early detection of sex in date palm. Primer BH-02
amplified an allele of 700 bp size which was unique to Medzool
genotypes and could distinguish them from all other varieties.
Analysis of polymorphism data using NTSYS-PC software,
grouped the 40 genotypes into 2 major clusters. Almost all the
genotypes of a particular variety were present together in their
corresponding groups. However, one female genotype Z2 was
outgrouped from all the other genotypes showing it to be the most
diverse. One male genotype (M8) showed 85% similarity to SH2
indicating it to be a male of Shamran or a close relative of it. Genetic
similarity based on ‘Simqual’ sub programme among various genotypes
ranged from 0.41 to 0.89% indicating moderate genetic variability
among genotypes. The average similarity between males and the thirty
females was found to be 0.69. Thus ISSR markers detected a high level
of polymorphism and the fingerprint data generated in this study can
be used for further cloning and sequencing of sex-linked markers in
date palm.
 
Date 2016-11-23T08:58:35Z
2016-11-23T08:58:35Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/87145
 
Language en
 
Format application/pdf
 
Publisher CCSHAU