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Molecular tagging and mapping of resistance to late leaf spot and rust in ground nut (Archis hypogea L.)

KrishiKosh

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Title Molecular tagging and mapping of resistance to late leaf spot and rust in ground nut (Archis hypogea L.)
 
Creator Yogendra.P.Khedikar
 
Contributor M.V.C.Gowda
 
Subject Genetic Plant Breeding
 
Description Groundnut (Arachis hypogaea L.) is an important oilseed crop of the world. Late leaf
spot (Phaeoisariopsis personata [(Berk. and Curt.) Deighton] and rust (Puccinia arachidis
Speg.) are the major destructive diseases of the crop. The use of resistant cultivars is the
preferred strategy to surmount the losses but resistance breeding has not been completely
successful. Molecular dissection of resistance traits using QTL analysis can improve the
efficiency of resistance breeding.
The present study was aimed at construction of genetic linkage map, identification of
markers / QTL associated with resistance to LLS and rust besides various yield related traits.
Two hundred and sixty eight RILs (TAG 24 × GPBD 4) segregating for late leaf spot and rust
were used to undertake QTL analysis. Phenotyping of the population was carried out under
artificial disease epiphytotics. Parents were assessed using 1089 SSR markers, of them 67
(6.15%) were found polymorphic. Single marker analysis revealed 34 markers associated
with LLS (1.12 to 5.78%) and 11 markers with rust (1.26 to 40.58%). Among the agronomic
traits, 45 markers were associated with contribution ranging from 1.1 to 10.23 per cent to
variation.
The study yielded first ever partial linkage map of the cultivated groundnut with 59
SSR markers on 13 LGs covering 909.40 cM (32%) and ten QTL for LLS (1.4 to 6.2%), one
QTL for rust (4.6 - 4.7%) and six QTL (3.2 - 11.3%) for different yield related traits with the
contribution of favorable alleles from GPBD 4 for resistance and TAG 24 for yield related
traits. The investigation revealed a need for further saturation of genetic map for complete
dissection of traits through QTL approach.
 
Date 2016-07-22T16:02:51Z
2016-07-22T16:02:51Z
2008
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69427
 
Format application/pdf
 
Publisher UAS Dharwad