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Gene Tagging of Fertility Restorer Gene for ARG Cytoplasm of Sunflower (Helianthus annuus L.) using RAPDs, ISSRs and Sunflower Specific SSRs

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Title Gene Tagging of Fertility Restorer Gene for ARG Cytoplasm of Sunflower (Helianthus annuus L.) using RAPDs, ISSRs and Sunflower Specific SSRs
 
Creator INDUMATHI BUTTA
 
Contributor Sreelakshmi, B
 
Subject Gene, Tagging, Fertility, Restorer, Gene, ARG, Cytoplasm, Sunflower, using, RAPDs, ISSRs, Sunflower, Specific, SSRs
 
Description Cytoplasmic male sterility is the most viable system for hybrid seed production in
sunflower. Till date, a single source of cytoplasm PET-1 has been used for hybrid seed
production in sunflower, which increases genetic vulnerability to pathogens. To
overcome this, there is a need for development of new cytoplasmic sources and
characterization of the restorers for use in hybrid breeding programs. In this regard, the
present investigation was carried out with the aim of genetic study of ‘ARG’ for fertility
restoration and to identify molecular markers linked to fertility restoration of the new
CMS derived from Helianthus argophyllus.
For the genetic study of fertility restoration, F2 and BC1F1 populations of ARG x
DSI 176 were used. By phenotypic fertility analysis, typical segregation patterns of 3:1
and 1:1 for fertile: sterile were obtained in F2 and BC1F1 populations respectively. Thus it
was confirmed that a single dominant gene was responsible for fertility restoration.
To identify molecular markers linked to male fertility restorer gene, the method of
Bulk Segregant Analysis was employed. Three marker systems viz., RAPD, ISSR and
SSR were used to identify markers closely linked to the gene. Screening the fertile and
sterile bulks with 571 RAPDs, 100 ISSRs and 120 SSRs, we found that an SSR marker
SF-19 was found to be linked to fertility restorer gene. Along with this, a partial fertility
restorer line with white pollen was taken, where, SF-19 failed to give polymorphism with
yellow and white pollen bulks indicating that the partial restorer do not carry the fr gene.
The yellow and white pollen bulks were constituted to identify molecular markers
linked to colour of the pollen. Among three marker systems used to screen bulks, one
RAPD marker OPU9_1200 was found to be linked to yellow pollen gene, which was
converted to sequence specific SCAR marker, which also confirmed at individual plant
level as well. Analysis by using 120 SSR markers, resulted in identification of 2 markers
ORS468 and ORS331 linked to yellow pollen gene. Interestingly these two markers were
located on the same linkage group (LG) 7. By interval mapping one more marker ORS
966 was found to be linked to yellow colour.
Thus, the SF-19 marker linked to fertility restorer gene can be used for the
development of new restorer lines through MAS. The SCAR marker developed and three
SSRs identified linked to colour of the pollen can be used to map some important traits
linked to colour of the pollen.
 
Date 2016-08-20T14:15:32Z
2016-08-20T14:15:32Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73189
 
Language en
 
Relation D7689;
 
Format application/pdf
 
Publisher ACHARYA N.G.RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD