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Phenetic and genetic analysis of abiotic stress tolerant Pigeon pea [Cajanus cajan (L.) Millspaugh] rhizobia retrieved from South-Western parts of Haryana

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Title Phenetic and genetic analysis of abiotic stress tolerant Pigeon pea [Cajanus cajan (L.) Millspaugh] rhizobia retrieved from South-Western parts of Haryana
 
Creator Kuldeep
 
Contributor Gera, Rajesh
 
Subject Cajanus cajan, PEG, MspI, HaeIII
 
Description Pigeon pea [Cajanus cajan (L.) Millspaugh] commonly called as toor dal or arhar dal is a
perennial member of the family Fabaceae. It is an important legume mainly grown as hardy and
drought tolerant crop. Environmental stress is a major component of natural selection in soil
ecosystem. Among several environmental conditions, the most prominent problematic abiotic factors in
the arid regions are drought and high temperature. For successful functioning of microbial
bioinoculants and their influence on plant growth and soil health, exhaustive efforts have been made to
expose microbial diversity, their distribution and behavior. Studies on molecular diversity of root
nodule bacteria in rhizosphere or nodules have been conducted to classify these rhizobia and also to
correlate with symbiotic properties, effectiveness and competitiveness of these bacteria. In the present
investigation, a total 196 rhizobial isolates were obtained from 84 soil samples collected from SouthWest Haryana using trap plant to study the diversity of rhizobia nodulating pigeon pea. All the
rhizobial isolates tested were able to grow at all the three temperatures i.e. 30, 35 and 40, except few
isolates where growth was slightly suppressed at 40
o
C. However, only 44 rhizobial isolates were able
to grow at 45
o
C, out of which some showed good, moderate and poor growth. Most of rhizobial isolates
were able to grow up to 20% PEG. However, there was drastic decrease in their growth rate with
increasing PEG concentration. Only, 71 and 3 rhizobial isolates were able to grow at 30 and 40% PEG,
respectively. All the selected rhizobial isolates were also characterized for IAA production, siderophore
production, P-solublization, ACC utilization and molecular characterization. The genomic DNA of all
these rhizobial isolates were isolated by CTAB method and tested for nodC and nifH gene primers and
hence authenticated as rhizobia. Genomic DNA of all the rhizobial isolates were also amplified with
16S rDNA gene primers using 27F and 1378R primers. The amplified product was subjected to RFLP
analysis with MspI and HaeIII restriction enzymes. Dendrogram based on 16S rDNA profiles showed
lot of diversity. Analysis of pigeon pea rhizobial isolates on ARDRA basis, 18 different biotypes were
formed at 80% similarity coefficient. Most prevalent biotype was 13
th
type, which prevails in all the
four districts. On the basis of abiotic stress tolerance, biochemical and molecular characterization, six
pigeon pea rhizobial isolates (PPH-8E, PPR-2, PPB-26A, PPM-30A, PPM-33C and PPM-37A) were
selected as most efficient rhizobial isolates.
 
Date 2016-09-09T14:47:47Z
2016-09-09T14:47:47Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76265
 
Language en
 
Format application/pdf
 
Publisher CCSHAU