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Immunological and Molecular Studies on Schistosomes of Cattle and Buffaloes

KrishiKosh

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Title Immunological and Molecular Studies on Schistosomes of Cattle and Buffaloes
 
Creator Sreenivasa Murthy, G.S.
 
Contributor Placid E. D'Souza
Suryanarayana, V.V.S.
Renukaprasad, C.
Sengupta, P.P.
Reddy, G.R.
Shrikrishna Isloor
 
Subject ---
 
Description Ph.D. Thesis
The present study on schistosomes of cattle and buffaloes was conducted by
screening bovine faecal samples by sedimentation and formal ether method. This
indicated 9.72 and 21.7 percent of S.spindale infection respectively. The modified
perfusion technique to recover live worms of S.spindale from mesenteric plexuses
indicated seven percent infection. Indirect ELISA with sera of cattle and buffaloes
revealed 18.40 and 16.0 percent of S.spindale infection respectively with the sensitivity
of 77.27 and specificity of 75 percent. Sandwich ELISA with 86 positive faecal samples
revealed sensitivity and specificity of 88.65 and 90.09 respectively.
SDS PAGE of S.spindale and S.indicum resulted in 12 and 11 polypeptides
respectively, of which 98, 66, 48 and 29 kDa were common major bands. In EITB, five
polypeptides of 66, 43, 34, 18 and 14 kDa in cattle and 43, 34 and 18kDa from buffaloes
showed intense immuno reactivity.
The PCR with 328 and 1088 bp products of three schistosoma species viz
S.spindale, S.indicum and S.nasale, amplified with mitochondrial genes of 12S rRNA
(rrns) and cytochrome oxidase sub unit I gene of isolates from Bangalore was performed.
The specificity was checked by NCBI nucleotide sequence data library with similar
partial sequences documented in pubmed.
Phylogenetic analysis of 12S ribosomal rRNA showed that S.spindale was
affiliated to distinct cluster of S.nasale from Bangladesh with 660 nucleotide
substitution. In COI gene, S.spindale Bangalore isolate and partial cds of
S. indicum fell in the same cluster with 100 percent homogeneity and divergence with
S. nasale and S. spindale of Bangladesh with nucleotide substitution of 960 & 800. It
was concluded that Indian schistosomes were distinct from Sri Lankan and Bangladesh
schistosomes.
 
Date 2016-08-02T12:39:49Z
2016-08-02T12:39:49Z
2011-11-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/70521
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar