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Genetic Studies of yield and its Component Traits and Identification of Molecular Markers Linked to Bacterial Wilt Resistance in Tomato (Solanum Lcopersicum L)

KrishiKosh

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Title Genetic Studies of yield and its Component Traits and Identification of Molecular Markers Linked to Bacterial Wilt Resistance in Tomato (Solanum Lcopersicum L)
 
Creator Ramesh Sharad. Mane
 
Contributor O. Sridevi
 
Subject Genetics & Plant Breeding
 
Description A study was conducted to understand the inheritance and identification of markers
associated with resistance to bacterial wilt in tomato. Among the 24 tomato genotypes
screened for bacterial wilt resistance, two highly resistant genotypes, CLN2768A and
CLN2777H and a highly susceptible Pusa Ruby were used in this study. F2 and backcross (B1
and B2) populations of crosses Pusa Ruby × CLN2768A and Pusa Ruby × CLN2777H were
used to study inheritance pattern of bacterial wilt resistance. In F2 population of both crosses,
segregation ratio 3 resistant: 1 susceptible was obtained which was confirmed in backcross
populations with 1:1 ratio. These results confirmed the role of single dominant gene for
governing the bacterial wilt resistance.
A total of 57 SSR and 79 RAPD markers were screened for parental polymorphism
and polymorphic markers were used for Bulked segregant analysis. BSA results confirmed a
single SSR marker in cross Pusa Ruby × CLN2768A and two SSR markers in cross Pusa
Ruby × CLN2777H putatively linked to bacterial wilt resistance gene. However, none of the
RAPD markers exhibited consistent polymorphism between resistance and susceptible bulks
of both crosses. Upon subjecting the F2 individuals for genotyping with the markers selected
in BSA, genetic association analysis indicated that SSR 20 marker linked to the bacterial wilt
resistance in both crosses. The SSR 20 marker can be used in future for marker assisted
selection of bacterial wilt resistance.
Generation mean analysis was done for understanding the nature and magnitude of
gene effects of different yield related characters. Epistasis was confirmed by scaling test and
joint scaling tests for all yield and yield components. The six generation mean analysis
showed the importance of dominant and dominant × dominant interaction in governing most
of the yield and yield components.
 
Date 2016-07-26T09:46:53Z
2016-07-26T09:46:53Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69828
 
Format application/pdf
 
Publisher UAS Dharwad