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SSR markers for resistance to Karnal bunt (Neovossia indica) in bread wheat (Triticum aestivum L.)

KrishiKosh

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Title SSR markers for resistance to Karnal bunt (Neovossia indica) in bread wheat (Triticum aestivum L.)
 
Creator Ravika
 
Contributor Chhabra, A. K.
 
Subject Wheat, Triticum aestivum, RILs, Karnal bunt and SSR markers
 
Description Present study was carried out to screen 85 recombinant inbred lines (RILs) of cross Aldan (resistant) / WH542 (susceptible) for Karnal bunt resistance and to identify putative SSR markers and validate known markers associated with Karnal bunt resistance in wheat. There was significant variation among all the recombinant inbred lines for percentage and coefficient of infection to Karnal bunt. Most of the RILs (53) and (57) showed up to 5% infection during the year 2012 and 2013 and thus were resistant. The percentage of infection (PI) in the susceptible RILs was up to 52.46% and 43.22% in the year 2012 and 2013 respectively, whereas, coefficient of infection on RILs was up to 21.30% and 17.46 % in the year 2012 and 2013, respectively. Analysis of variance revealed considerable magnitude of variability among different RILs for all morphological traits studied namely, plant height, spike length, days to flowering, days to maturity, tillers/plant, 1000 grain weight and grain yield per meter row. A total of 45 SSR markers were used to screen parental genotypes out of these 15 were amplified. These 15 SSR markers were used to screen 20 selected RILs and a fingerprinting database was generated. Out of these 15, 6 were polymorphic and had presence of bands of different molecular weights for resistant and susceptible lines. Genetic similarity analysis showed that parental genotypes were quite distinct from each other. The cluster analysis led to distribution of parents and their RILs in to two groups at the similarity coefficient value of 0.31. Major cluster I, included resistant parent Aldan with resistant lines except one susceptible line in this group and major cluster II had susceptible parent WH542 and susceptible lines. Bulk segregant analysis showed that three primers (Xgwm637, Xgwm337, Xgwm538) which were already reported to be associated with Karnal bunt resistance, also showed association with Karnal bunt resistance in population under study. These three markers were further used to screen on whole population and results were confirmed. Hence, these markers may be used for marker assisted selection for Karnal bunt resistance in early segregating generations of wheat.
 
Date 2016-08-16T15:35:07Z
2016-08-16T15:35:07Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72647
 
Language en
 
Format application/pdf
 
Publisher CCSHAU