ICAR Research Data Repository for Knowledge Management
“DEVELOPMENT OF IMPROVED TECHNOLOGY FOR MASS MULTIPLICATION OF Trichoderma spp. a BIOFUNGICIDE”
KrishiKosh
View Archive Info| Field | Value | |
| Title |
“DEVELOPMENT OF IMPROVED TECHNOLOGY FOR MASS MULTIPLICATION OF Trichoderma spp. a BIOFUNGICIDE”
M.Sc. (PLANT PATHOLOGY) |
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| Creator |
Shirole, Sanjay
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| Contributor |
Awadhiya, G.K.
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| Subject |
DEVELOPMENT, MASS MULTIPLICATION, Trichoderma spp. a BIOFUNGICIDE
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| Description |
The present investigation entitled “Development of Improved Technology for Mass Multiplication of Trichoderma spp. a Biofungicide” was carried out in the Department of Plant Pathology, College of Agriculture, IGKV, Raipur (C.G.) Seven isolates of Trichoderma viride were isolated from soil collected from different locations and commercial product available in the market. Among T. viride isolates, Tricho-5 isolate was selected for further studies. T. viride (Tricho-5) inhibitated the growth of soil borne pathogens like Sclerotium rolfsii by 78% followed by Fusarium oxysporum f. sp. lini by 76.72% and Rhizoctonia solani by 73.38%. Different media, temperature, colour of light, pH studies revealed that PDA medium supported best growth and sporulation of the fungi. The optimum temperature for growth and sporulation was recorded at 20-300C. The radial growth of T. viride (Tricho-5) was significantly higher in plates covered with black sheet placed in dark shade and sporulation designated at good in blue and transparent colored light. Similarly, excellent growth occurred at pH 5 to 7. Glucose was superior carbon source for growth and sporulation of T. viride (Tricho-5) followed by Sucrose and Dextrose. Ammonium chloride was superior nitrogen source for growth and sporulation of T. viride (Tricho-5) followed by Ammonium sulphate and Ammonium nitrate. Among seven isolates of T. viride, Tricho-2 followed by Tricho-1 showed maximum acid phosphatase activity. Several grains, organic waste materials and potato dextrose broth were evaluated as substrates for mass multiplication of antagonistic fungi T. viride (Tricho-5). Among the substrates, potato dextrose broth, Mung grains and Sugarcane baggages proved to be most promising for growth and sporulation of the fungi. In farmyard manure, multiplication of the fungi was favoured when the moisture content of the substrate was maintained between 40 and 60%. By mixing the nucleus inoculum of the antagonistic fungi developed in FYM incubating up to one month, a field inoculum could be prepared. Neither fumigation of substrate with formaldehyde prior to inoculation nor covering it with polyethylene sheets after inoculation had any beneficial effect on multiplication of antagonistic fungi. |
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| Date |
2016-09-22T11:16:18Z
2016-09-22T11:16:18Z 2010 |
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| Type |
Thesis
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| Identifier |
124p.
http://krishikosh.egranth.ac.in/handle/1/78315 |
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| Language |
en
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| Format |
application/pdf
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| Publisher |
INDIRA GANDHI KRISHI VISHWAVIDYALYA, RAIPUR
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