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Molecular characterization of horsegram yellow mosaic virus and its management

KrishiKosh

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Title Molecular characterization of horsegram yellow mosaic virus and its management
 
Creator G. U, Prema
 
Contributor Nagaraju
 
Subject diseases, biological phenomena, dna, proteins, viruses, genetic structures, genes, planting, genotypes, nucleotides
 
Description Horsegram yellow mosaic virus is one of the major constraints for cultivation of
horsegram in peninsular India, transmitted by white fly, Bemisia tabaci. The complete
DNA-A component (2.7 kb) of HgYMV was cloned and sequenced. Comparison of the
complete DNA-A sequence of HgYMV-GKVK-Bangalore with other bipartite
begomoviruses revealed 98-95 per cent identity with HgYMV isolates from Tamil Nadu
and Srilanka, respectively. Phylogenetic analyses revealed that DNA-A component of
HgYMV-GKVK-Bangalore clustered with HgYMV-Tamil Nadu:HG and HgYMVSrilanka:
FB isolates. Complete analysis of DNA-A sequence of HgYMV-GKVKBangalore
isolate showed presence of six open reading frames (ORFs) similar to that of
DNA-A component of other begomoviruses. The coat protein gene (CP) of YMV isolates
infecting different legumes was successfully amplified using specific primers.
Phylogenetic analysis, nucleotide sequence and amino acid sequence comparisions of
coat protein gene indicated that HgYMV, FBYMV, SBYMV, LBYMV and PBYMV
belong to one group of yellow mosaic viruses whereas MYMV and BGYMV belong to
another group of begomoviruses. Among one hundred horsegram genotypes screened
during 2011, 37 were free from the disease. Four genotypes showed highly resistant
reaction (TCR-1291, TCR -1100, TCR -8-A, TCR -1433). Eight (TCR -1016, TCR -
1056, TCR -172-A, TCR -232-A, TCR -1290, TCR -95-A, TCR -1214, TCR -2) showed
resistant reaction. Out of 110 horsegram germplasm lines screened during 2012, none
were free from the disease. Five genotypes viz., AK-38, HG-GP, DPI-2278, Paiyur-1 and
Paiyur-2 recorded highly resistant reaction and only three genotypes were resistant
(PHG-9, AK-42, BGM-1) reaction. Host range studies of HgYMV revealed that the virus
could infect pole bean, french bean, ring bean, lima bean and soybean and not greengram,
blackgram, cowpea, pigeon pea, yard long bean, field bean, moth bean, rice bean and
cluster bean. Silicon and nanosilver were tried against the virus and found ineffective in
the management of HgYMV as compared to insecticides.
 
Date 2016-05-20T14:47:39Z
2016-05-20T14:47:39Z
2013-09-02
 
Type Thesis
 
Identifier Th-10626
http://krishikosh.egranth.ac.in/handle/1/66177
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru