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EVALUATION OF CYANOBACTERIA FOR MYCOSPORINE LIKE AMINOACIDS

KrishiKosh

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Title EVALUATION OF CYANOBACTERIA FOR MYCOSPORINE LIKE AMINOACIDS
M.Sc.
 
Creator YATTAPU PRASAD REDDY
 
Contributor G. Abraham
 
Subject cyanobacteria, amino acids, acidity, productivity, enzymes, nitrogen, environment, inorganic acid salts, nostoc, imports
 
Description Random soil samples have been collected from Rann of Kutch area from the Kutch District of Gujarat from Western India. The soil samples were used for the isolation of cyanobacteria and the morphological and cultural characteristics were used for identification. The identified strains belong to Anabaena, Nostoc, Phromidium, Westiellopsis and Synechocystisand were maintained at various salinity levels. The morphological identity was further confirmed using 16S rDNA technique and all the strains examined showed an amplification product of 1500 bp. BLAST analysis was performed and it showed close similarity with cultured cyanobacterial species. The isolates were screened for the presence of mycosporine like amino acids and out of the ten isolates seven were found positive for the presence of MAAs. Heterocystous forms produced more MAAs as compared to non-heterocystous forms. Growth as well as the cellular constituents of the cyanobacterial species producing mycosporine like amino acids showed variability. Positive correlation was found to exist between cellular constituents and MAA content in the isolates. Similar trend was observed by the enzymes of nitrogen assimilation such as nitrate reductase, glutamine synthetase and nitrogenase.
Two promising strains such as YPR-9 (Nostocsp) and YPR-3 (Phormidium) were selected for further analysis on the basis of high MAA content. On the basis of retention time and absorption spectra through HPLC studies presence of mycosporine like amino acids was confirmed in these strains. It was observed that the pH, temperature, salinity and the nitrogen sources influenced the productionof mycosporine like amino acids in the selected cyanobacterial strains such asNostoc and Phormidium. The present study thus led to the identification of cyanobacterial strains from the extreme environment of Rann of Kutch. Mycosporine like amino acid production could be enhanced through modulation of external factors such as pH, temperature, salinity and nitrogen source. The promising strains in terms of MAA production could be further investigated and exploited for enhanced production of these compounds.
 
Date 2016-03-11T16:27:19Z
2016-03-11T16:27:19Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65096
 
Language en_US
 
Format application/pdf
 
Publisher IARI, Division of Microbiology, New Delhi