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IDENTIFICATION OF SHEEP AND GOAT SPECIES BY RAW MEAT USING PCR ASSAY

KrishiKosh

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Title IDENTIFICATION OF SHEEP AND GOAT SPECIES BY RAW MEAT USING PCR ASSAY
 
Creator PANWAR, NAIMUDDIN
 
Contributor G.C. Gahlot
 
Subject rhizobium, yields, peas, planting, sowing, diseases, fertilizers, application methods, nutrients, biofertilizers
 
Description Adulteration of high quality meat and meat products with their inferior/cheaper counterparts has become a common practice in the meat industry. Many consumers are concerned about the meat they eat and accurate labeling is important to help fair-trade, and to enable consumers to make informed choices. The present study was carried out to develop a simple, reliable, single step and highly specific PCR assay to identify goat and sheep species in raw meat. Sensitivity of PCR was also determined to identify particular meat species from mixture of meats. Genomic DNA was isolated from meat samples of sheep, goat and buffalo as per method described by Ausubel et al. (1987) with some modifications. Mitochondrial DNA fragment of cyt b and D-Loop region was amplified by conventional PCR using a common forward primer and speciesspecific reverse primers of goat and sheep as described by Matsunaga et al. (1999), Kumar et al. (2011) and Karabasanavar et al. (2011). PCR was optimized with respect to annealing temperature to give species specific amplification. A common forward and goat specific reverse primer amplified 157 bp fragment with goat meat DNA while common forward with sheep specific reverse primer amplified 331 bp fragment with sheep meat DNA. The goat specific forward and reverse primer amplified 294 bp fragments with goat meat DNA while sheep specific forward and reverse primer amplified 404 bp fragments with sheep meat DNA. PCR did not produce any cross specific amplification with buffalo meat DNA. Both or any one of mtDNA fragment (cyt b and D-Loop) region in animals could be used to differentiate meat species. The PCR could detect particular meat in concentration as low as 1% from mixture of meats. Developed duplex PCR assay is simple, rapid, highly sensitive and specific without cross-reactivity. It has the potential to be developed into a laboratory tool for species identification especially for goat and sheep traceability.
Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001
 
Date 2016-12-27T14:29:16Z
2016-12-27T14:29:16Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/93257
 
Language en
 
Format application/pdf
 
Publisher Rajasthan University of Veterinary and Animal Sciences, Bikaner - 334001