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Microanatomical Studies On The Parotid And Mandibular Salivary Glands In Sheep (Ovis aries)

KrishiKosh

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Title Microanatomical Studies On The Parotid And Mandibular Salivary Glands In Sheep (Ovis aries)
 
Creator Muthukrishnan, S
 
Contributor Basha, Sabiha Hayath
Ramesh, Geetha
Ushakumary, S
Kumar, R. Suresh
 
Description Histogenesis, Histomorphology and Histochemical studies of parotid and
mandibular salivary glands in sheep was conducted in different prenatal and postnatal
age groups. For developmental studies, the sheep foetuses were collected from one to
three months of age. Tissue pieces from neonatal, young and adult were collected in
neutral buffered formalin, formal alcohol and Bouin’s fixative and processed for
routine histological staining. Paraffin and cryosections of fixed and unfixed tissues
were used for histology and histochemical study. Transmission electron microscopic
study of parotid and mandibular salivary glands was done in young and adult sheep.
The primordial anlage of parotid salivary gland was first noticed as an epithelial
invagination from the stomodeal ectoderm near the labiogingival groove in 25 day –old
sheep foetus while the primordia of mandibular salivary gland was first noticed in
23day-old sheep foetus as an epithelial aggregation that sprouted from the
linguogingival lamina. Sequential changes in the development of both the glands were
recorded.In all the age groups studied, the parotid and mandibular salivary glands were
surrounded by a connective tissue capsule from which septa traversed and divided the
glandular parenchyma into lobes and lobules.The parotid gland of sheep was
characterized by purely serous acini. Each acini comprised of four to six pyramidal
cells with basal spherical nuclei and granulated eosinophilic cytoplasm.
The serous acini were moderately positive for neutral mucin and strongly
positive for acid mucin. The serous cells showed moderate metachromasia and were
negative for fucose while the ducts were positive for fucose, mannose and galactose.
The parenchyma of the parotid gland showed positive reaction for proteins, lipids and
enzymes.
In the present study, the mandibular salivary gland comprised of mixed acini
with more number of mucous and few serous acini. The mucous acini were surrounded
by crescent shaped serous demilunes. The mucous acini composed of five to six
pyramidal cells enclosing a narrow lumen. The nuclei was flattened and located in the
basal part of the cytoplasm.
The mucous acini were strongly positive for PAS and Best carmine while the
serous acini and demilunes were alcian blue positive and carmine positive. The mucous
acini were negative for fucose and moderately positive for mannose and galactose. The
parenchyma of the mandibular salivary gland showed positive reaction for proteins,
lipids and enzymes.
In the present study, the duct system of both the glands comprised of
intercalated, striated, interlobular, lobar and main excretory duct. All the ducts were
positive for alcian blue, Ninhydrin Schiff and enzymes.The intercalated duct and acini
of both the glands were surrounded by myoepithelial cells with varying morphology.
The ultrastructural detail of both parotid and mandibular salivary glands was recorded
in young and adult age groups.
 
Date 2016-05-31T11:36:56Z
2016-05-31T11:36:56Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66472
 
Language en
 
Format application/pdf
 
Publisher Tamil Nadu Veterinary and Animal Sciences University