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Molecular detection and characterization of banana bunchy top virus and somaclonal variants in tissue culture banana plants

KrishiKosh

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Title Molecular detection and characterization of banana bunchy top virus and somaclonal variants in tissue culture banana plants
 
Creator W. A. R. T, Wickramaarachchi
 
Contributor K. T, Rangaswamy
 
Subject bananas, dna, planting, pcr, biological phenomena, viruses, proteins, diseases, tissue culture, productivity
 
Description In the recent years, banana viral diseases are effectively
managed by using virus free tissue culture planting materials. Virus
indexing of large number of tissue culture planting materials indicated
the presence of viruses in very low percentage. Field survey of banana
plantations for prevalence of viruses revealed the low incidence of viral
diseases in banana crops grown using tissue culture plants compared
to sucker grown plantations. However, the incidence of off types
ranging from 1.3 to 24.0 per cent was observed in banana crop
established using tissue culture plants. Dwarf off type was the most
frequent off type observed and contributed to 70 per cent of the total
off types recorded.
RAPD primer OPJ-04 which supported the amplification of an
approximate product of 1.5 kb from DNA extracted from normal plants
but not from dwarf plants was found suitable for detecting dwarf off
types from micropropagated banana cv. Grand Naine. Multiplex PCR
technique with a SCAR marker and an internal control was developed
to increase the reliability of the detection of dwarf off types.
The specific polyclonal antibodies produced against bacterially
expressed coat protein of BBTV showed strong reaction with crude sap
from infected plant tissues in western blot, dot blot immunoassay and
DAC-ELISA. The antiserum had a titre of 1:2000.
Multiplex PCR technique was developed for parallel detection of
both BBTV and BSV using virus specific primers. A Novel, rapid and
cost effective technique was developed for mass indexing of tissue
culture banana samples for BBTV with a relatively less number of PCR
reactions using combinatorial screening format. A PCR technique for
detection of BBTV in viruliferous aphids was standardized using the
template DNA extracted from minimum of five aphids with “Jalali”
DNA extraction method.
Analysis of sequences of major genomic components of
Bangalore and Sri Lankan isolates of BBTV showed clustering of both
isolates together with other Indian isolates in South Pacific group.
High degree of genetic homology of Sri Lankan isolate with Bangalore
isolate and with other isolates from India suggested a close genetic
relationship of Sri Lankan and Indian BBTV isolates.
 
Date 2016-05-26T10:41:16Z
2016-05-26T10:41:16Z
2011-11-18
 
Type Thesis
 
Identifier Th-9939
http://krishikosh.egranth.ac.in/handle/1/66332
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru