ICAR Research Data Repository for Knowledge Management
Molecular characterization and genetic divergence analysis in date palm (Phoenix dactylifera L.) using RAPD markers
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Molecular characterization and genetic divergence analysis in date palm (Phoenix dactylifera L.) using RAPD markers
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| Creator |
Rekha Rani
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| Contributor |
Kharb, Puspha
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| Subject |
Datepalm, RAPD, polymorphism, Phoenix dactylifera
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| Description |
Date palm (Phoenix dactylifera L.) is a long living dioecious evergreen with 2n=36. It has the distinction of being one of the oldest fruit trees in the world. It is an important plantation crop due to nutritive quality of its fruit. This study was undertaken to assess polymorphism among different date palm genotypes (including male and female genotypes). Molecular polymorphism in 40 date palm genotypes (10 male and 30 female) was assessed by using 29 RAPD primers. A total of 223 amplicons were observed out of which 3 were monomorphic and 220 were polymorphic. Thenumber of amplified DNA bands varied between 2 and 17 with an average of 7.69±0.70 bands per primer. Average polymorphism across the 40 genotypes was 99.12±0.62. Three primers were identified which produced unique alleles capable of differentiating female l genotypes of Medjool from rest of the genotypes. Analysis of the polymorphism profile generated using NTSYS-PC software, grouped the 40 genotypes into 2 major clusters and thirteen sub clusters. Z2 and M6 were out grouped showing them to be genetically most diverse among all genotypes used in present study. Based on ‘Simqual’ sub programme, genetic similarity was calculated. It ranged from 0.28 to 0.93 indicating moderate genetic variability among the genotypes. The average similarity of male plants with all the thirty female plants was found to be 0.7. Thus RAPD markers detected a high level of polymorphism and the fingerprint data generated in this study can be used for the identification of Medjool variety of date palm. |
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| Date |
2016-11-23T09:16:56Z
2016-11-23T09:16:56Z 2007 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/87154
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| Language |
en
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| Format |
application/pdf
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| Publisher |
CCSHAU
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