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Linkage mapping of quantitative trait loci for root morphology and selection of desirable genotypes from aerobic x lowland indica crosses in rice (Oryza sativa L.)

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Title Linkage mapping of quantitative trait loci for root morphology and selection of desirable genotypes from aerobic x lowland indica crosses in rice (Oryza sativa L.)
 
Creator Kharb, Anju
 
Contributor Jain, Rajinder Kumar
 
Subject Unable to Generate Tags ruth elizabeth ekka , t-2776.pdf
 
Description Identification of stable QTL for traits promoting aerobic adaptation using molecular markers can
greatly enhance the efficacy of breeding programs to develop water-efficient, high yielding, aerobic
rice varieties. Filial (F2 and F3) and backcross ( BC2F2 and BC1F2) populations derived from the crosses
between high-yielding low-land (HKR47) and aerobic (MAS26 and MASARB25) indica rice varieties,
displayed large variation for various physio-morphological traits including grain yield per plant and
root traits (in case of net house evaluation). Phenotypic correlation analysis showed positive correlation
(r = 0.279 and 0.351) between grain yield per plant and root length in both F2 and F3 population and
between grain yield per plant and fresh & dry root weight (r = 0.232 and 0.269) in F2 population. Under
aerobic field conditions, Grain yield per plant showed significant positive correlation with plant height,
effective number of tillers/plant and panicle length in all the four populations. A total of 803 SSR
markers, distributed on 12 rice chromosomes, were analyzed for polymorphism in two parental rice
genotypes (HKR 47 and MAS 26); of these 125 (about 16%) displayed polymorphism. SSR
fingerprint databases of three HKR47 x MAS26 F2 and F3 populations (94 F2 in net house, 42 F3 in net
house and 52 F3 plants in field) were prepared using 125, 88 and 88 polymorphic SSR markers,
respectively. NTSYS-pc UPGMA tree cluster analysis and two-dimensional PCA scaling showed
scattering of the F2 and F3 populations between the two distinct parental genotypes; the populations
were inclined towards MAS26. Composite interval mapping (CIM) analysis by Win QTL cartographer
2.5 revealed a total of six QTLs (qPN8.1, qPN8.2, qPN8.3, qTGW8.1, qYPP8.1 and qRL8.1) in HKR47 x
MAS26 F2 population, grown in the net house on chromosome 8 (within a region of 24.9 cM) which
individually explained 13.7-27.3% of the phenotypic variation. In case of HKR47 x MAS26 F3
population, based on field data and net house data, two (qTGW6.1 and qLB8.1) and six QTL (qPH3.1,
qLB8.1, qTGW6.1, qTGW11.1, qRT8.1 and qRT8.2) were mapped, respectively. As many as 20 F3, 6 BC2F2
and 5 BC1F2 promising plants having higher grain yield and/or better root length/biomass were
selected. Data on monitoring of putative QTL identified showed that out of twenty selected plants, four
plants had 13 QTL, three plants had 12 QTL and two plants had 11 QTL in homozygous or
heterozygous condition.
 
Date 2016-02-06T12:19:54Z
2016-02-06T12:19:54Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/64193
 
Language en
 
Format application/pdf
 
Publisher CCSHAU