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“Standardization of embryo rescue technique under sub-tropical conditions and use of DNA markers for hybrid identification in grapes (Vitis vinifera L.)

KrishiKosh

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Title “Standardization of embryo rescue technique under sub-tropical conditions and use of DNA markers for hybrid identification in grapes (Vitis vinifera L.)
 
Creator Nripendra Vikram Singh
 
Contributor A. K. Singh
 
Subject weeds, control methods, herbicides, groundnuts, yields, crops, planting, developmental stages, drying, biological development
 
Description t-8045
Seedless grapes suffer from stenospermocarpy so, technique of embryo rescue
employing in-ovulo culture has been adopted in grape breeding in western world. Thus,
by adopting in-ovulo embryo rescue in grape breeding programme, about 7-8 years can
be curtailed for developing superior seedless cultivars. Furthermore, the hybridity of the
progenies can also be confirmed at an early stage of development by using Simple
Sequence Repeats (SSR) markers. With respect to mean ovule age (days after
pollination), maximum mean percent green ovules (47.75), ovule maturity (28.75%) and
ovule-embryo growth (2.13 mm2
) were obtained when ovules were cultured at 24 days
after pollination. The concentration of IAA (4mg/l) + GA3 (0.5mg/l) proved to be most
effective for germination (13.84 %) and ovule maturity (26.67%). Among various
media used for culturing ovules, ovules on MS medium (1/2 macro + 1/1 micro)
required minimum days to germinate (96.67) and registered highest germination
(13.75%). Chilling treatment was proved to be one of the important factors for embryo
maturation and 60 days of chilling treatment at 4 C was found better than 45 days. With
respect to the effect of different growth regulators on various rooting and shooting
parameters, IBA (1.0 mg/l) and NAA (1.5 mg/l) were found superior. While comparing
different hardening strategies, glass jar with polypropylene cap (GPP) was found to be
most effective as far as days to hardening (35.33) as well as hardening success (66.67%)
were concerned. Among the various strains used for hardening of rescued plantlets T3
inoculated plantlets registered highest plant height (19.59 cm) and survival percent
(94.00), T2 was found to be the best for phenol synthesis, irrespective of hybrids and
treatments (9.10 µg/g fresh wt) and T1 inoculated hybrid plantlets synthesized
maximum amount of chlorophyll (3.24 mg/g) and produced highest shoot fresh weight:
dry weight ratio (9.52) as well as highest root fresh wt: dry wt. ratio (9.82)
Out of twenty SSR primers used most of them have shown polymorphism in
parental genotypes. The primers which were able to show polymorphism among all four
parental genotypes are namely, WS2, WMD5, WMD7, WMD28, WMD32 and
SS7VrZAGA67. The percent polymorphism ranged from 0% to 100% with six primers
showing 100% polymorphism and the band amplification produced by them ranged
between 100 to 300 bp.
 
Date 2017-01-03T09:54:12Z
2017-01-03T09:54:12Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94043
 
Format application/pdf