Record Details

EVALUATION AND BIOCHEMICAL ESTIMATION OF GREENGRAM VARIETIES INFECTED BY ROOT-KNOT NEMATODE, Meloidogyne incognita

KrishiKosh

View Archive Info
 
 
Field Value
 
Title EVALUATION AND BIOCHEMICAL ESTIMATION OF GREENGRAM VARIETIES INFECTED BY ROOT-KNOT NEMATODE, Meloidogyne incognita
 
Creator Pandey, Ritu Kumari
 
Contributor Nayak, D.K
 
Subject EVALUATION AND BIOCHEMICAL ESTI GREENGRAM VARIETIES INFECTED ROOT-KNOT NEMATODE, Meloidogyne incognita
 
Description Nematode infection interrupts biological processes in plants manifested as nutritional
deficiencies or excesses, stunting, wilting and reduced yields Meloidogyne incognita is a sedentary
parasite that modify the host tissues (Hussey and Williamson, 1996) and biochemical environment
(Gommers and Dropkin,1982) to meet its nutritional requirements (Dorhout et al., 1993). This
nematode species decreases the photosynthetic rate by decreasing the level of potassium in the
leaves. It also leads to the reduction of nitrogen concentration and or other elements that reduces
the physiological activities of the plants resulting reduced yield (Melakeberhan et al., 1987). It
was also reported by Devrajan et al., in 2003 that the photosynthetic efficiency and total
chlorophyll contents were reduced in plants inoculated with Meloidogyne incognita.
The experiment was laid out in green house of Department of Nematology and pots were
arranged in Complete Randomised Design with three replications for chemical analysis. The present
study aimed at determining the change in the leaf nutrient contents of different resistant and
susceptible cultivars/varieties of greengram against root-knot nematode, Meloidogyne incognita. For
this study four resistant cultivars, i.e., 24 ML-233,7 GGG 10-14,17 IPM 9901-6 and 8 GM 04-02
and two susceptible cultivars 28 PM 10-12 and 29 PUSA 0672 were accessed for investigation of
nitrogen ,phosphorus and potassium contents and also for chlorophyll content, total sugar contents,
phenol contents, crude protein and proline contents of both infected and healthy plants. Under pot
culture condition in green house, Leaf Nitrogen and Phosphorus contents were determined by
Kjeldahl extraction method and Potassium content were determined by atomic absorption
spectrophotometer. Maximum Nitrogen content of 196.61% was noted in variety 7 GGG 10-14
while minimum as 88.57% in variety 28 PM 10-12.Similarly Phosphorus maximum 46.49 % noted
in variety 29 PUSA 0672 and minimum 52.26% in variety 28 PM 10-12 .
The changes in nutrient profile in plants inoculated with nematodes cause reduction in
chlorophyll contents that ultimately leads to reduction in photosynthetic efficiency. Maximum
chlorophyll ‘a’ 28.30 mg/g in variety 17 IPM 9901-6 and minimum chlorophyll ‘a’ -96.36 mg/g in
variety 7 GGG 10-14.Similarly maximum chlorophyll’b’76.66 mg/g was found in variety 17 IPM
9901-6.The total chlorophyll content was maximum 86.27 mg/g in variety 17 IPM 9901-6 and
minimum 54.92 mg/g in variety 29 PUSA 0672.
The study had been conducted to know the effects of root-knot nematode, M. incognita
infestations on alterations in biochemical metabolities in 45 days old plants. Quantitative analysis
of total sugar, crude protein, phenol and proline contents in both healthy and infected plants
showed a greater concentration in infected plants over healthy one by 39,4.10 , 146.15 and 125
percentage increase respectively in both the host plants. It can be concluded that altered
biochemical reactions in diseased tissues perhaps results in enhanced metabolism after the
infection of parasite. Similarly for proline content, total sugar content, phenol content of both
healthy and infected were studied. The proline was maximum as 125% in variety 29 PUSA 0672
and minimum 7.69% in variety 28 PM 10-12.The increase % in phenol was maximum 146.15%
in variety 29 PUSA 0672 and decrease % was maximum 23.88% in variety 8 GM 04-02.The total
sugar content was decreased maximum 39% in variety 8 GM 04-02 and minimum 61.78 % in
varieties 28 PM 10-12.The decrease of crude protein content was maximum 4.10 % in the variety
8 GM 04-02 .
Hydrolysis of reserved chemical metabolities is carried out by their respective enzymes to
cater the growth and multiplication of parasites and providing susceptibility to the host and results
in reduced growth and low yield of plant products.
 
Date 2017-01-04T15:26:41Z
2017-01-04T15:26:41Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94517
 
Relation Th;
 
Format application/pdf