In silico molecular docking, pharmacophore and 3D QSAR studies on Mer receptor tyrosine kinase inhibitors
KrishiKosh
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Title |
In silico molecular docking, pharmacophore and 3D QSAR studies on Mer receptor tyrosine kinase inhibitors
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Creator |
Mishra, Adyasha
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Contributor |
Pradhan, S K
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Subject |
Receptor Tyrosine Kinases, pharmacophore, 3D-QSAR, HBA, HBD
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Description |
Mer is a member of TAM (Tyro3, Axl, Mer) subfamily of Receptor Tyrosine Kinases, the abnormal activation or overexpression of which has been implicated in survival signalling of many human cancers. Inhibition of this Mer kinase may enhance the sensitivity of cancer cell to cytotoxic agents and would potentially be a therapeutic strategy to target cancer cells. By characterizing different lead inhibitors, the researchers had solved different crystal structures of these Mer kinases with and without inhibitors, which is beneficial in the field of Structure Based Drug Design of Mer kinase inhibitors. In silico molecular docking with three dimensional pharmacophore and quantitative structure-activity relationship (3D-QSAR) studies have been carried out using different sets of inhibitors of Mer kinase. The binding pose of different inhibitors at active site amino acid residues of Mer RTK can be observed from the docking studies. From the results of Rigid docking using Glide XP, top compounds from each set were subjected to Induced fit docking, which show the compound 8 and 2 from the first set and Compound 44 and 43 from the second set have good docking score, glide energy and good interactions in comparison with other compounds as well as the co-crystal. The pharmacophore and 3D QSAR studies have resulted in five point pharmacophore models with one hydrogen bond acceptor (HBA), one hydrogen bond donor (HBD), one hydrophobic group(H) two aromatic rings(R) are used to derive a predictive atom-based 3D-QSAR model. The best alignment was with Compound 20 and the good co-relation co-efficient and other values with the training and test sets are R2=0.985, SD=0.160, F=423.1, Q2=0.634, RMSE=0.632, Pearson-R=0.825. The results indicate that compound 20 is a good inhibitor and further discovery of novel lead molecules for the inhibition of Mer RTK and can be done from the proposed pharmacophore model. |
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Date |
2017-01-04T14:15:01Z
2017-01-04T14:15:01Z 2015 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/94458
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Language |
en
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Relation |
Th;4346
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Format |
application/pdf
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