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INFLUENCE OF INSECTICIDES ON THE AChE ACTIVITY IN SPODOPTERA LITURA FABRICIUS

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Title INFLUENCE OF INSECTICIDES ON THE AChE ACTIVITY IN SPODOPTERA LITURA FABRICIUS
 
Creator SUVARNA, KALVA
 
Contributor SRIDEVI, D
 
Subject irrigation, soil management, groundwater table, yields, crops, area, land resources, drainage systems, saline soils, fruits
 
Description Studies were conducted in the Department of Entomology, College of Agriculture,
Rajendranagar, Hyderabad to determine the relative toxicity of selected organophosphate
insecticides viz., profenofos and chlorpyriphos and carbamates viz., thiodicarb and
methomyl against third instar larvae of Spodoptera litura ( Fabricius) of local and
Nalgonda populations by topical application method. Enzymatic studies were conducted in
the Department of Pharmacology and Toxicology, College of Veterinary Science,
Rajendranagar, Hyderabad to determine AChE activity in the populations of S. litura larvae
treated with LC50 by the method of Ellman et al . (1961).
LC50 values based on bioassay studies indicated that among four insecticides,
chlorpyriphos exhibited greater toxicity by topical application method after 24 hrs of
exposure. The relative toxicity of these insecticides against local population was found to
be in the order of chlorpyriphos (1.00) > thiodicarb (0.60) > profenofos(0.58) > methomyl
(0.30). While against Nalgonda population it was chlorpyriphos (1.00) > thiodicarb (0.68)
> profenofos (0.60) > methomyl (0.56) after taking the toxicity of chlorpyriphos as check
as unity.
The mean AChE activity in μmoles/min/ml based on enzyme assay studies showed
the highest AChE activity (1.266) in control followed by profenofos (0.988) and lowest
AChE activity (0.628) for thiodicarb in Local population, while, the values were 1.387,
1.120, and 0.688, respectively for Nalgonda population.
The mean AChE activity in μ moles/min/ml of sample and the change in per cent
AChE activity in the sample containing the selected insecticides viz., profenofos,
chlorpyriphos, thiodicarb and methomyl for a period of 30 min was gradually decreased
with the progress of time in both the populations.
The per cent AChE activity was the highest for profenofos with 78.8 % with
respect to control and lowest (48.7 %) for thiodicarb in Local population, while for
Nalgonda population the values were 81.1 % and 49.3 % , respectively.
Positive correlation between the toxicity and AChE activity was observed for
organophosphate insecticides and no correlation was observed in case of carbamate
insecticides.
 
Date 2016-06-07T14:25:47Z
2016-06-07T14:25:47Z
2011
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66955
 
Language en
 
Relation D8851;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY