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Molecular Characterization of Rabies Virus and Diagnosis of Rabies by Rt-Pcr in Animals

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Title Molecular Characterization of Rabies Virus and Diagnosis of Rabies by Rt-Pcr in Animals
 
Creator Chandrashekhara, N.
 
Contributor Rathnamma, D.
Mukhopadhyaya, H.K.
Shrikrishna Isloor
Veeregowda, B.M.
Yathiraj, S.
Satyanarayana, M.L.
Suguna Rao
 
Subject ---
 
Description Ph.D. Thesis
A total of 124 rabies suspected brain samples collected from five different animal
species spread across five geographically distinct states viz., Karnataka, Tamil Nadu,
Kerala, Maharashtra and Andhra Pradesh were subjected to FAT, dRIT and conventional
RT-PCR to arrive at confirmatory diagnosis of rabies. Further, in the study forty nine
saliva samples for ante mortem diagnosis and fifty two skin samples for post mortem
diagnosis from rabies suspected animals were also collected and subjected for RT-PCR
respectively. Ninety three of 124 samples turned positive both by FAT and dRIT,
whereas 97 rabies suspected brain samples were found positive by RT-PCR. Only eight
out of 49 saliva samples and seven out of 52 skin samples turned positive by RT-PCR for
ante mortem and post mortem diagnosis of rabies respectively. Twenty one RT-PCR
amplicons including positive control vaccine virus amplicon were chosen for nucleotide
sequencing and comparison of sequences for phylogenetic analysis. Phylogenetic analysis
of a portion of N gene of 21 RABV nucleotide sequences from southern states of India in
comparison to other Indian and exotic RABV and RRVs revealed that RABV nucleotide
sequences from southern India exist as a single genetic cluster irrespective of region and
species of origin and are related to one another and vaccine strain clustered with Pasteur
and Challenge Virus Strains. The analysis also revealed the close relationship of RABV
nucleotide sequences from Kerala and Tamil Nadu to the Sri Lankan RABV variants.
Further, in the study Indian RABV nucleotide sequences were not found to be related to
RRVs.
 
Date 2016-07-22T15:16:30Z
2016-07-22T15:16:30Z
2013-05-09
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69379
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar