Molecular Characterization of Rabies Virus and Diagnosis of Rabies by Rt-Pcr in Animals
KrishiKosh
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Title |
Molecular Characterization of Rabies Virus and Diagnosis of Rabies by Rt-Pcr in Animals
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Creator |
Chandrashekhara, N.
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Contributor |
Rathnamma, D.
Mukhopadhyaya, H.K. Shrikrishna Isloor Veeregowda, B.M. Yathiraj, S. Satyanarayana, M.L. Suguna Rao |
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Subject |
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Description |
Ph.D. Thesis
A total of 124 rabies suspected brain samples collected from five different animal species spread across five geographically distinct states viz., Karnataka, Tamil Nadu, Kerala, Maharashtra and Andhra Pradesh were subjected to FAT, dRIT and conventional RT-PCR to arrive at confirmatory diagnosis of rabies. Further, in the study forty nine saliva samples for ante mortem diagnosis and fifty two skin samples for post mortem diagnosis from rabies suspected animals were also collected and subjected for RT-PCR respectively. Ninety three of 124 samples turned positive both by FAT and dRIT, whereas 97 rabies suspected brain samples were found positive by RT-PCR. Only eight out of 49 saliva samples and seven out of 52 skin samples turned positive by RT-PCR for ante mortem and post mortem diagnosis of rabies respectively. Twenty one RT-PCR amplicons including positive control vaccine virus amplicon were chosen for nucleotide sequencing and comparison of sequences for phylogenetic analysis. Phylogenetic analysis of a portion of N gene of 21 RABV nucleotide sequences from southern states of India in comparison to other Indian and exotic RABV and RRVs revealed that RABV nucleotide sequences from southern India exist as a single genetic cluster irrespective of region and species of origin and are related to one another and vaccine strain clustered with Pasteur and Challenge Virus Strains. The analysis also revealed the close relationship of RABV nucleotide sequences from Kerala and Tamil Nadu to the Sri Lankan RABV variants. Further, in the study Indian RABV nucleotide sequences were not found to be related to RRVs. |
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Date |
2016-07-22T15:16:30Z
2016-07-22T15:16:30Z 2013-05-09 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/69379
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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