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Morphological and molecular characterization of rose species

KrishiKosh

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Title Morphological and molecular characterization of rose species
M.Sc.
 
Creator Henuka Rai
 
Contributor Raju V.S. Dantuluri
 
Subject Rosa species, Dendrogram, Cluster analysis, Principal component analysis
 
Description T-8813
Interspecific hybridization in roses (Rosa spp.) has generated thousands of cultivars with commercial value, but only ten species are commonly represented in the pedigrees of modern cultivars. This would suggest that unexploited wild rose species have the potential to broaden the overall gene pool of rose breeding programs in general. In the present study Random amplified polymorphic DNA analysis was employed for the molecular characterization of the 23 rose genotypes. Out of the 50 random primers used, 26 primers amplified the scorable banding patterns. These 26 primers gave 168 polymorphic bands out of 169 bands analyzed. The percentage polymorphism ranged from 66.6% to 100%. R. moschata and R. tomentosa showed highest similarity with 0.91 similarity coefficient index and R. lutea and R. nitida x R. rugusa showed the maximum diversity with similarity coefficient 0.42. The dendrogram obtained by Jaccard‟s similarity coefficient method distributed 23 species into 4 major clusters. In agreement with Rehder‟s classification, members of Synstylae section, R. brunonii, R. multiflora and R. wichurana fell in the same cluster, also the member of the Section Indicae, R. indica var. odorata, R. indica major, R. chinensis viridiflora and R. bourboniana fell in the same cluster. However all the three varieties of R. damascena did not fall into same cluster. R. hybrida has shown some similarity with R. damascena (Himroz) as they fell into same cluster. R. rubrifolia was independent from rest of the rose genotypes at 0.59 similarity coefficient. The clusters based on RAPD analysis clearly demonstrate the existence of genetic variation within the 23 species.
Keywords: Random Amplified Polymorphic DNA, rose species, cluster analysis, similarity coefficient.
 
Date 2016-08-17T13:23:29Z
2016-08-17T13:23:29Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/72737
 
Format application/pdf
 
Publisher IARI, Division of Floriculture and Landscaping