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ASSESSMENT OF MOLECULAR DIVERSITY AMONG ADVANCED BREEDING LINES OF RICE (Oryza sativa L.) VARIETY - SAMBA MAHSURI

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Title ASSESSMENT OF MOLECULAR DIVERSITY AMONG ADVANCED BREEDING LINES OF RICE (Oryza sativa L.) VARIETY - SAMBA MAHSURI
 
Creator STEFFI GRAF, DEVATHALA
 
Contributor SURESH, J
 
Subject ASSESSMENT, MOLECULAR, DIVERSITY, ADVANCED, BREEDING, LINES, RICE, VARIETY, SAMBA MAHSURI
 
Description Rice (Oryza sativa L.) is the most important staple food grain of all cereal crops
and feeds more than half of the world’s population. Advanced breeding lines are pre released
lines which include homozygous lines, mutant lines and the lines derived from biotechnology
programmes contains valuable gene combinations. These constitute an important source of
genetic variation for utilization in breeding of high yielding rice varieties and hybrids.
The present study was undertaken with an objective to assess the genetic diversity
among advanced breeding lines (mutant lines - M4 generation derived from Samba mahsuri
variety, which showed earlier tolerance to yellow stem borer(YSB) infestation.The study was
also intended to identify some allele specific markers (Molecular Ids). Out of the total 60 SSR
markers distributed across 12 chromosomes, tested, 21 were found to be polymorphic and were
used to assess the extent of genetic diversity among 42 mutant lines of samba mahsuri
A total of 44 alleles were detected across 42 mutant lines of samba mahsuri by 21
polymorphic SSR markers. The number of alleles generated per locus by each marker ranged
from 2 to 3 with an average of 2.09 number of alleles per locus.
Polymorphism information content (PIC) values among the SSR loci tested ranged from
0.565(RM562) detected on chromosome 1 to 0.816 (RM 26213) on chromosome 11 with an
average of 0.685 per locus.
Cluster analysis performed by using UPGMA based on similarity co-efficient grouped 42
mutant lines into two major clusters with 37% dissimilarity. Cluster I consisted of 41 breeding
lines which showed 35% dissimilarity and was further subdivided into IA and IB. IA and IB
consisted of 40 lines and 1 mutant line, respectively with 26% dissimilarity comprised of 30 and
8 mutant lines. Cluster II consisted of 1 mutant line.
Out of 21 polymorphic SSR markers, four SSR markers were identified as allele specific
markers, viz., RM485 on chromosome 2, RM6487 on chromosome 4, RM1359 on chromosome 4
and RM26998 on chromosome 11. The specific markers (Molecular Ids) identified in the present
study will also be used for identification of particular mutants. The loci which undergone
mutations in particular mutants needs to be associated with morphological characters like
resistance reaction to the YSB (Yellow Stem Borer ) to know clearly which loci corresponds to
the altered phenotype. The hypothesis of EMS mutation which causes the point mutation in the
genome was verified and evidenced from this study.
 
Date 2016-09-07T13:49:45Z
2016-09-07T13:49:45Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/75923
 
Language en
 
Relation D9515;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD