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Aqueous extracts of marine invertebrates from Cuba coastline display neutral aminopeptidase inhibitory activities and effects on cancer cells and <em>Plasmodium falciparum</em> parasites

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Title Statement Aqueous extracts of marine invertebrates from Cuba coastline display neutral aminopeptidase inhibitory activities and effects on cancer cells and <em>Plasmodium falciparum</em> parasites
 
Added Entry - Uncontrolled Name Pascual Alonso, Isel ; 1- Center for Protein Studies. Faculty of Biology. University of Havana
Bounaadja, Lotfi ; 2-Molécules de Communication et Adaptation des Microorganismes (MCAM, UMR 7245), Sorbonne Universités, Muséum national d’Histoire naturelle, CNRS, CP52, 57 rue Cuvier 75005 Paris (France)
Sánchez, Laura ; 1- Center for Protein Studies. Faculty of Biology. University of Havana
Rivera, Laura ; 1- Center for Protein Studies. Faculty of Biology. University of Havana
Tarnus, Céline ; 3: Laboratoire de Chimie Organique et Bioorganique – COB, Institut de Recherche Jean-Baptiste Donnet, 3 bis rue Alfred Werner - 68093 Mulhouse Cedex - France
Schmitt, Marjorie ; 4: Laboratoire de Chimie Moléculaire - CNRS UMR7509, Institut de Recherche Jean-Baptiste Donnet, 3 bis rue Alfred Werner - 68093 Mulhouse Cedex – France
Garcia, Gabriela ; 1- Center for Protein Studies. Faculty of Biology. University of Havana
Diaz, Lisset ; 1- Center for Protein Studies. Faculty of Biology. University of Havana
Hernandez- Zanuy, Aida ; 5: Institute of Oceanology, AMA, CITMA, Cuba
Sánchez, Belinda ; 6:Center of Molecular Immunology, BioCubaFarma, Cuba.
Florent, Isabelle ; 2: Molécules de Communication et Adaptation des Microorganismes (MCAM, UMR 7245), Sorbonne Universités, Muséum national d’Histoire naturelle, CNRS, CP52, 57 rue Cuvier 75005 Paris (France)
IUBMB Mid-Career fellowship program to support the research stay of Dr. Isel Pascual at MCAM, UMR 7245, 2014 and French ANR project MAMMAMIA “design of potential anti MAlarial M1/M17 AMinopeptiIdase Agents”, 2012-2016.
 
Uncontrolled Index Term marine biotechnology; enzymes; peptidase inhibitors
Cancer, Inhibitors, Malaria, Marine invertebrates, Neutral aminopeptidases, Screening
 
Summary, etc. Neutral aminopeptidases are enzymes distributed in all living organisms. By hydrolyzing biologically active peptides in tissues and biological fluids, they are involved in the control of many physiological processes. They became established targets for new therapeutic agents in cancer, but also in parasitic diseases like malaria. Marine organisms are promising sources for biomolecules but few examples of neutral aminopeptidase inhibitors are described. The goal of this work was to search in Cuban marine invertebrates, for inhibitory activities of neutral aminopeptidases of biomedical relevance, belonging to the M1 and M17 metallopeptidase families. The screening of inhibitory activities was performed using aqueous crude extracts and their 2.5 % TCA treatments. The treatments with 2.5 % TCA increased the recovery of inhibitory activities versus all enzymes tested and from all of marine species. These inhibitory activities were dose-dependent in all cases, with certain selectivity for PfA-M17 regarding hLAP, and good inhibition of hAPN. Interestingly, some TCA treated extracts displayed promising effect on either Plasmodium parasite growth as well as on PC3 and 3LL cells. This contribution is the first report identifying inhibitory activities from marine invertebrates, directed against human and malarial neutral aminopeptidases, suggesting a potential for biomedical applications for the corresponding marine species.
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2017-08-16 07:59:10
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/14931
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 8, ##issue.no## 2 (2017): June 2017
 
Language Note en
 
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