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Micropropagation and <em>Agrobacterium rhizogenes</em> mediated transformation studies in <em>Mucuna pruriens</em> (L.) DC.

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Title Statement Micropropagation and <em>Agrobacterium rhizogenes</em> mediated transformation studies in <em>Mucuna pruriens</em> (L.) DC.
 
Added Entry - Uncontrolled Name Vishwakarma, K S; North Maharashtra University, Jalgaon.
Mohammed, S I; North Maharashtra University, Jalgaon.
Chaudhari, A R; North Maharashtra University, Jalgaon.
Salunkhe, N S; North Maharashtra University, Jalgaon.
Maheshwari, V L; North Maharashtra University, Jalgaon.
Authors acknowledges financial support from UGC, New Delhi under UGC major research project. Authors are also grateful to UGC and DST for making available the research facilities under the UGC-SAP and DST-FIST programme sanctioned to the School of Life S
 
Uncontrolled Index Term Plant tissue culture
Acetosyringone, Agrobacterium rhizogenes, Hairy root, Manual wounding, Micropropagation, Mucuna pruriens (L.) DC.
 
Summary, etc. An efficient protocol for micropropagation and <em>Agrobacterium rhizogenes</em> mediated transformation has been standardized for <em>Mucuna pruriens </em> (L.) DC. For micropropagation, shoot tips from aseptically germinated seedlings were inoculated on MS medium supplemented with different concentrations (2–20 µM) of phytohormones. Of the various phytohormones tried, kinetin (14 µM) resulted in 4 shoots/explant after four weeks of culture. Rooting was best obtained on half-strength MS medium supplemented with 6 µM concentration of NAA. Regenerated plants were hardened with 75% of survival frequency. For hairy root induction,different explants were infected by two strains of Agrobacterium rhizogenes (MTCC 2364 and MTCC 532) and effect of manual wounding and acetosyringone was studied. Of the two strains tried, MTCC 2364 showed hairy roots induction in leaf explant only by manual wounding method. While in case of acetosyringone (50-250 µM), mediated method hairy root induction was observed in all the explants studied except leaf. Out of the two strains used, MTCC 2364 showed better results in terms of roots emerged/explant and number of days required for root induction as compared to the strain MTCC 532.
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2017-08-16 07:59:10
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/13321
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 8, ##issue.no## 2 (2017): June 2017
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJNPR/article/download/13321/38789
http://op.niscair.res.in/index.php/IJNPR/article/download/13321/38790
 
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