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STRUCTURAL & FUNCTIONAL INSIGHTS INTO METHIONINE ABC TRANSPORTER IN BACILLUS MEGATERIUM

KrishiKosh

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Title STRUCTURAL & FUNCTIONAL INSIGHTS INTO METHIONINE ABC TRANSPORTER IN BACILLUS MEGATERIUM
 
Creator Pradhan, Smruti Priyambada
 
Contributor Rath, S N
 
Subject BACILLUS MEGATERIUM, METHIONINE, protein-protein interaction, RING analysis
 
Description Bacillus megaterium is the biggest known gram positive bacteria. Methionine ABC transporter of B. megaterium uses the energy of ATP binding cassette. ATP binding cassette (ABC) systems are widely distributed among all living organisms. Bacterial ABC transporter are essential in cell viability, virulence and pathogenicity. ABC importers are found mostly in bacteria. The major function of ABC import system is to provide essential nutrients to bacteria. Bacillus megaterium is about 2 times greater in volume compared to that of an E.coli. The larger size has allowed several protein to be successfully studied. The protein (metN ABC transporter) was subjected to phylogeny analysis using the MEGA and clustal omega tools. The 3D structure of the protein was generated through discovery studio 3.5 server. The DS 3.5
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generated 100 structures of the protein, out of which the structure that had the lowest DOPE score (-35610.918) was selected for further analysis. The 3D protein structure was further validated through server PROCHECK, QMEAN, ProSA, ERRAT and Verify 3D. The docking analysis was carried out by Autodock 1.5.6. Molecular dynamics (MD) simulations study was carried out to characterize its structural and dynamical features at 30 ns in explicit solvent using the GROMACS version 5.0.7 server. MD simulation showed the proposed model retains its stable conformation in aqueous solution. The protein-protein interaction was carried out using string database to understand the intricate molecular mechanism and for recognition novel modulator and also to forecasts a confidence score. The RING web portal was used to find out the residue-residue interaction of the protein structure. The RING analysis shows that the connection of the protein interaction network is close & its interaction type is multiple.
 
Date 2017-01-04T12:35:53Z
2017-01-04T12:35:53Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94396
 
Language en
 
Relation Th;4641
 
Format application/pdf