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Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3.

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Relation http://ir.cftri.com/12744/
http://dx.doi.org/10.1080/21501203.2017.1281358
 
Title Molecular profiling and antioxidant as well as anti-bacterial potential of
polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3.
 
Creator Ananda, Danagoudar
Chandrashekhar, G. Joshi
Sunil Kumar, R.
Anu Appaiah, K. A.
 
Subject 04 Fungi
32 Antioxidants
 
Description Fungal endophytes are less studied group of microorganisms with vast therapeutic potential. A
polyphenol producing endophyticfungus was isolated from Zingiber officinale rhizome. The ethyl
acetate extract of Aspergillus austroafricanus (EAE) was tested against five human pathogenic
bacteria by disc diffusion method. Antioxidant activity of EAE was determined by DPPH, H2O2 and
nitric oxide radical-scavenging methods. DNA protection from the OH radicals was tested by
agarose gel electrophoresis. High-performance liquid chromatography was used to determine
the total yield of polyphenols. The identity of the endophytic fungus was established as A.
austroafricanus CGJ-B3 (GenBank accession No. KT780617) based on rDNA and phylogenetic
analysis. EAE showed significant antioxidant, antimicrobial activity and DNA damage protection
capacity. The HPLC analysis showed the presence of polyphenols such as p-coumaric acid, ferulic
acid and cinnamic acid and the content was about 0.392 ± 0.08 μg/mg, 4.35 ± 0.16 μg/mg and
1.976 ± 0.11 μg/mg, respectively. A. austroafricanus CGJ-B3 isolated from Z. officinale is a
promising potential pharmaceutical agent and can be used as an alternative source of polyphenols
like p-coumaric acid, ferulic acid and cinnamic acid.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/12744/1/MYCOLOGY%2C%202017%20VOL.%208%2C%20NO.%201%2C%2028%E2%80%9338.pdf
Ananda, Danagoudar and Chandrashekhar, G. Joshi and Sunil Kumar, R. and Anu Appaiah, K. A. (2017) Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3. Mycology, 8 (1). pp. 28-38.