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Glucose-rich diet aggravates monocrotophos-induced dopaminergic neuronal dysfunction in Caenorhabditis elegans.

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Relation http://ir.cftri.com/12761/
http://dx.doi.org/10.1002/jat.3426
 
Title Glucose-rich diet aggravates
monocrotophos-induced dopaminergic
neuronal dysfunction in Caenorhabditis elegans.
 
Creator Salim, Chinnu
Rajini, P. S.
 
Subject 26 Pesticide Chemistry
17 Toxicology
 
Description The present study aimed to obtain insights into the mechanism(s) by which glucose-rich diet aggravates
monocrotophos (MCP)-induced dopaminergic neuronal dysfunction in Caenorhabditis elegans. In this study, we exposed three
different strains of worms (wild-type N2, CB1112 (cat-2(e1112)II, tyrosine hydroxylase-deficient mutant, catecholamine absent)
and the transgenic BZ555 (egls1-dat-1p::green fluorescent protein [GFP]) (in which bright GFP is tagged to the dopamine neuronal
soma and processes) grown and maintained in normal nematode growthmedium or 2% glucose enriched-nematode growth
mediumtoMCP (0.75mM) for 48 h. After the exposure, dopamine-mediated behaviors such as repulsion to nonanone, chemotaxis
index and basal slowing response were determined in worms. Dopamine, 3,4-dihydroxy phenyl acetic acid and homovanillic acid
content were quantified in N2 worms. The extent of neurodegeneration was visualized and quantified in dat-1::GFPworms. Basal
slowing response study clearly indicated that cat-2 worms exposed to MCP and glucose were less affected compared to N2 of the
same treatment. Learning andmemory were affected byMCP and glucose.While MCP-treated worms showed lesser repulsion to
nonanone compared to control worms, MCP-treated, glucose-fed worms showed a greater reduction in repulsion to nonanone.
Further, MCP-treated, glucose-fed worms exhibited a marked reduction in dopamine content and an increase in 3,4-dihydroxy
phenyl acetic acid and homovanillic acid levels compared to that in control. Dat-1::GFP showed a significant degeneration of
dopaminergic neurons when exposed to glucose and MCP. Thus, our results clearly demonstrate that glucose-rich diet
aggravates the dopaminergic neuronal dysfunction induced by MCP in C. elegans.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/12761/1/J.%20Appl.%20Toxicol.%202017%3B%2037%20772%E2%80%93780.pdf
Salim, Chinnu and Rajini, P. S. (2017) Glucose-rich diet aggravates monocrotophos-induced dopaminergic neuronal dysfunction in Caenorhabditis elegans. Journal of Applied Toxicology, 37. pp. 772-780.