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Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship.

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Relation http://ir.cftri.com/12777/
http://dx.doi.org/10.1016/j.carbpol.2016.12.046
 
Title Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship.
 
Creator Sabeeta, Kapoor
Shylaja, M. Dharmesh
 
Subject 28 Polysaccharide Chemistry
03 Tomato
 
Description tPectic Polysaccharide (PP) from dietary sources has been known to prevent cancer growth and henceimpede cancer progression. We evaluated anticancer effect of Pectic-Oligosaccharide isolated from SourRaw Tomato (SrTPO); its bioavailability and structure elucidated from purified fraction (SrTPO1). SrTPO1inhibited galectin-3 activity with MIC of 0.25 ug/mL (100 fold better than standard galactose), inhibitedthe growth of AGS cells (IC503.4 �g/mL) and induced apoptosis (70% inhibition at 30 �g/mL concentra-tion). Normal– NIH 3T3 cells were not affected by SrTPO as opposed to doxorubicin, a known anticancerdrug, which reduced 76% viability at equivalent dose. SrTPO1 was identified as RhamnogalacturonanI-arabinogalactan (RGI-AG), where repeated alternative rhamnose and galacturonic acid residues wereobserved while arabinose in the branch point and �-1,4 linked galactose in the linear chain form. SrTPOwas found to be bioavailable as evaluated by FITC labelled oligos inside the cell, which was in reciprocalproportion with apoptosis.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/12777/1/Carbohydrate%20Polymers%20160%20%282017%29%2052%E2%80%9361.pdf
Sabeeta, Kapoor and Shylaja, M. Dharmesh (2017) Pectic Oligosaccharide from tomato exhibiting anticancer potential on a gastric cancer cell line: Structure-function relationship. Carbohydrate Polymers, 160. pp. 52-61.