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A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity

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Title A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity
 
Creator Soror, Sameh H.
Verma, V.
Rao, Ren
Rasool, Shafaq
Koul, S.
Qazi, G. N.
Cullum, John
 
Subject Biological Sciences
 
Description The genome sequence of Streptomyces coelicolor
A3(2) contains 51 putative lipase and esterase genes
mostly of unknown function. The gene estB (locus SCO
6966) was expressed as a His-tagged protein in E. coli.
Esterase B was active at low temperatures exerting its maximum activity at 30°C and retaining more than 25% of its
activity at 4°C. The optimum pH was 8–8.5. The enzyme
was active against short synthetic p-nitrophenylesters
(C2–C10) with maximum activity towards the acetate ester
(C2). The esterase was tested on 13 series of racemic esters
of potential interest for the synthesis of chiral pharmaceutical compounds. 4 of the series were substrates and a modestdegree of enantioselectivity was observed (enantiomeric ratios of 1.1–1.9).
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/183/1/10.1007s10295-007-0224-6.pdf
Soror, Sameh H. and Verma, V. and Rao, Ren and Rasool, Shafaq and Koul, S. and Qazi, G. N. and Cullum, John (2007) A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity. Journal of Industrial Microbiology & Biotechnology, 34 (8). pp. 525-531. ISSN 1367-5435
 
Relation http://dx.doi.org/10.1007/s10295-007-0224-6
http://iiim.csircentral.net/183/