Record Details


Field	Value

Title	Extra thin alginate film: an efficient technique for protoplast culture

Creator	Sharma, Madhu Pati, P K Ahuja, Paramvir Singh

Subject	Plant sciences Plant Biotechnology

Description	This paper reports an efficient protoplast culture technique, the “extra thin alginate film’’ technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. The efficiency of this technique was evaluated with two model plant systems, viz., Nicotiana tabacum and Lotus corniculatus, and a comparison was made with the “thin alginate layer’’ technique, another efficient protoplast culture system. Results indicate that the culture technique with extra thin alginate film is as efficient as the technique with thin alginate layer, with many additional advantages. The present innovation overcomes most of the limitations of protoplast culture techniques described so far and can now be applied to a wide variety of crops to check its general applicability.

Date	2005

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://ihbt.csircentral.net/28/1/2005_Extra_thin_alginate.pdf Sharma, Madhu and Pati, P K and Ahuja, Paramvir Singh (2005) Extra thin alginate film: an efficient technique for protoplast culture. Protoplasma, 226 (3-4). pp. 217-221.

Relation	http://ihbt.csircentral.net/28/

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