Record Details

A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of <i>Cuminum cyminum</i> seeds

IR@CSIR-IIIM

View Archive Info
 
 
Field Value
 
Title A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of Cuminum cyminum seeds
 
Creator Sharma, S. N.
Johri, R. K.
Sharma, S. C.
Suri, K. A.
Gupta, B. D.
Khan, I.
Sharma, P.
Sharma, V. K.
Dutt, P.
Amina, M.
Satti, N. K.
 
Subject Chemical Sciences
 
Description In this paper we describe a sensitive and reproducible reversed-phase highperformance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1-O-β-D-galacturonide-4′-(1-O-β-D-glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1, from the seeds of Cuminum cyminum. Compound 1 was separated isocratically on a C18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC–mass spectrometry and by spectroscopic techniques(1H and 13C NMR). The purity of 1 was also confirmed by HPTLC.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/195/1/10.1556AChrom.21.2009.3.12.pdf
Sharma, S. N. and Johri, R. K. and Sharma, S. C. and Suri, K. A. and Gupta, B. D. and Khan, I. and Sharma, P. and Sharma, V. K. and Dutt, P. and Amina, M. and Satti, N. K. (2009) A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of <i>Cuminum cyminum</i> seeds. Acta Chromatographica, 21 (3). pp. 499-512. ISSN 1233-2356
 
Relation http://dx.doi.org/10.1556/AChrom.21.2009.3.12
http://iiim.csircentral.net/195/