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HPTLC Determination of Swertiamarin and Amarogentin in Swertia Species from the Western Himalayas

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Title HPTLC Determination of Swertiamarin and Amarogentin in
Swertia Species from the Western Himalayas
 
Creator Bhandari, P
Singh , Bikram
Gupta , A P
Kaul , V K
 
Subject Natural Product Chemistry
 
Description A high-performance thin-layer chromatographic (HPTLC) method
has been established for simple and rapid quantification of two secoiridoids, swertiamarin and amarogentin, in two Swertia species. HPTLC of secoiridoids was performed on precoated silica 60 F254 plates with EtOAc–MeOH–H2O, 77 + 8 + 8 (v/v), as mobile phase. Densitometric determination of the secoiridoids was performed at λ = 235 nm in reflectance/absorbance mode. The method was validated
for precision, repeatability, and accuracy. Average recovery
of amarogentin and swertiamarin was 94.5 and 96.5%, respectively, showing that the reproducibility of the method was excellent. The method is simple, precise, specific, sensitive, and accurate and can be used for routine quality control of raw materials and herbal formulations.
 
Publisher Research Inst Medicinal Plants , PO BOX 11, H-2011 BUDAKALASZ, Hungary
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/51/1/2006_HPTLC_Determination.pdf
Bhandari, P and Singh , Bikram and Gupta , A P and Kaul , V K (2006) HPTLC Determination of Swertiamarin and Amarogentin in Swertia Species from the Western Himalayas. JPC-Journal of Planar Chromatography-Modern TLC , 19 (109). pp. 212-215. ISSN 0933-4173
 
Relation http://ihbt.csircentral.net/51/