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SOMATIC EMBRYOGENESIS FROM IMMATURE ZYGOTIC EMBRYOS OF ROSA BOURBONIANA DESP.†

IR@CSIR-IHBT

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Title SOMATIC EMBRYOGENESIS FROM IMMATURE ZYGOTIC EMBRYOS OF ROSA BOURBONIANA DESP.†
 
Creator Kaur, N
Pati, P K
Sharma, Madhu
Ahuja, Paramvir Singh
 
Subject Nutraceuticals
 
Description A protocol for the induction of somatic embryogenesis from immature zygotic embryos of Rosa bourboniana, a scented rose
species, was established. Somatic embryos were induced after 8 wk of inoculation of zygotic embryos on MS medium
supplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (5–15mM). In addition to 2,4-
dichlorophenoxy acetic acid concentrations, somatic embryogenesis was also influenced by the month of collection of the
explant and the stage of maturity of the hip. Maximum embryogenic response (16.6%) was observed using 2,4-
dichlorophenoxy acetic acid (15mM), from green hips in the month of September. The use of L-proline (800 mg l21) was
found to be optimum for secondary embryogenesis. On periodic subculturing, the cultures formed somatic embryos
sustainably over a period of 18 mo. For somatic embryo germination, 6-benzylaminopurine (5 mM) was found to be most
suitable. Rooted plants were transferred successfully to soil and appear morphologically normal under greenhouse
conditions. Transfer of plants for hardening was most suitable during the active growth period between June and September.
 
Date 2007
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/56/1/2006_Somatic.pdf
Kaur, N and Pati, P K and Sharma, Madhu and Ahuja, Paramvir Singh (2007) SOMATIC EMBRYOGENESIS FROM IMMATURE ZYGOTIC EMBRYOS OF ROSA BOURBONIANA DESP.†. Cellular and Development Biology Plant, 42 (2). pp. 124-127.
 
Relation http://ihbt.csircentral.net/56/