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Influence of soil reaction on diversity and antifungal activity of fluorescent pseudomonads in crop rhizospheres

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Title Influence of soil reaction on diversity and antifungal activity of fluorescent pseudomonads in crop rhizospheres
 
Creator Verma, R
Naosekpam, AS
Kumar, Sanjay
Prasad, R
Shanmugam, V
 
Subject Plant Biotechnology
 
Description The diversity and antifungal activity of fluorescent pseudomonads isolated from rhizospheres of tea, gladiolus, carnation and black gram grown in acidic soils with similar texture and climatic conditions were studied. Biochemical characterisation including antibiotic resistance assay, RAPD and PCR-RFLP studies revealed a largely homogenous population. At soil pH (5.2), the isolates exhibited growth with varying levels of siderophore production, irrespective of crop rhizospheres. Two isolates with maximum chitinase production showed antagonism. The bacterial populations in general lacked the ability to produce deleterious traits such as cellulase, pectinase and hydrogen cyanide. However, increased pH levels beyond 5.2 caused reduction in metabolite production with reduced antifungal activity. The homogeneity of the bacterial population irrespective of crop rhizospheres together with decreased secondary metabolite production at higher pH levels reinstated the importance of soil over host plant in influencing rhizosphere populations. The studies also yielded acid tolerant chitinase producing antagonistic fluorescent pseudomonads.
 
Publisher Elsevier Sci Ltd , The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1GB, Oxon, England
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/74/1/2007_Influence.pdf
Verma, R and Naosekpam, AS and Kumar, Sanjay and Prasad, R and Shanmugam, V (2008) Influence of soil reaction on diversity and antifungal activity of fluorescent pseudomonads in crop rhizospheres. Bioresource Technology, 98 (7). pp. 1346-1352. ISSN 0960-8524
 
Relation http://ihbt.csircentral.net/74/