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In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting

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Title In vitro production of Indian citrus ringspot virus (ICRSV)
free kinnow plants employing phytotherapy coupled
with shoot tip grafting
 
Creator Sharma , S
Singh , B
Rani , G
Zaidi, A A
Hallan, Vipin
Nagpal , A K
Virk , G S
 
Subject Plant sciences
Plant Biotechnology
 
Description This paper reports the elimination of Indian
citrus ringspot virus (ICRSV) from “kinnow” (Citrus
nobilis Lour × Citrus deliciosa Tenora) employing
phytotherapy coupled with shoot tip grafting under in
vitro conditions. Nodal segments from infected mother
plant (indexed by indirect enzyme-linked immunosorbent
assay [ELISA] and reverse transcriptase PCR [RT-PCR])
were cultured on Murashige and Skoog medium containing
2iP (1 mg/l or 4.9 μM) and malt extract (800 mg/l)
along with different concentrations of aqueous extracts
from leaves of Azadirachta indica (Neem), Sorghum
vulgare (Jowar), and roots of Boerhaavia diffusa (Punarnava). Shoot tips were excised from the nodal sprouts and grafted on to rough lemon (Citrus jambhiri) under aseptic conditions. Maximum effect (50% virus elimination) was seen for aqueous leaf extracts of A. indica followed by B. diffusa root extract (42.86%) and S. vulgare leaf extract
(31.58%). Plants/plantlets were considered virus-free only
when showing negative reactions by both indirect ELISA
and RT-PCR.
 
Publisher Springer, Van Godewijckstraat 30, 3311 GZ Dordrecht, The Netherlands .
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/78/1/2007_In_vitro_production_of.pdf
Sharma , S and Singh , B and Rani , G and Zaidi, A A and Hallan, Vipin and Nagpal , A K and Virk , G S (2008) In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting. Plant Cell,Tissue And Organ Culture, 92. pp. 374-379. ISSN 0167-6857
 
Relation http://ihbt.csircentral.net/78/