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Complete nucleotide sequence analysis of Cymbidium mosaic virus Indian isolate: further evidence for natural recombination among potexviruses

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Title Complete nucleotide sequence analysis of Cymbidium mosaic
virus Indian isolate: further evidence for natural recombination among potexviruses
 
Creator Sherpa, A R
Hallan, Vipin
Pathak, P
Zaidi, A A
 
Subject Plant Biotechnology
 
Description The complete nucleotide sequence of an Indian strain of Cymbidium mosaic virus (CymMV) was determined and
compared with other potexviruses. Phylogenetic analyses on the basis of RNA-dependent RNA polymerase (RdRp),
triple gene block protein and coat protein (CP) amino acid sequences revealed that CymMV is closely related to the
Narcissus mosaic virus (NMV), Scallion virus X (SVX), Pepino mosaic virus (PepMV) and Potato aucuba mosaic
virus (PAMV). Different sets of primers were used for the amplifi cation of different regions of the genome through
RT-PCR and the amplifi ed genes were cloned in a suitable vector. The full genome of the Indian isolate of CymMV
from Phaius tankervilliae shares 96–97% similarity with isolates reported from other countries. It was found that the
CP gene of CymMV shares a high similarity with each other and other potexviruses. One of the Indian isolates seems
to be a recombinant formed by the intermolecular recombination of two other CymMV isolates. The phylogenetic
analyses, Recombination Detection Program (RDP2) analyses and sequence alignment survey provided evidence for
the occurrence of a recombination between an Indian isolate (AM055720) as the major parent, and a Korean type-2
isolate (AF016914) as the minor parent. Recombination was also observed between a Singapore isolate (U62963) as
the major parent, and a Taiwan CymMV (AY571289) as the minor parent.
 
Publisher Indian Academy of Science, C V Raman Avenue, Sadashivanagar, P B #8005, Bangalore 560 080, India
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/80/1/2007_Complete.pdf
Sherpa, A R and Hallan, Vipin and Pathak, P and Zaidi, A A (2008) Complete nucleotide sequence analysis of Cymbidium mosaic virus Indian isolate: further evidence for natural recombination among potexviruses. Journal of Biosource, 34 (4). pp. 663-669. ISSN 0250-5991
 
Relation http://ihbt.csircentral.net/80/