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Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity

IR@CSIR-IIIM

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Title Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity
 
Creator Chaubey, Asha
Parshad, Rajinder
Koul, Surrinder
Taneja, Subhash C.
Qazi, Ghulam N.
 
Subject Biological Sciences
 
Description Arthrobacter sp. lipase (ABL, MTCC no. 5125)is being recognized as an efficient enzyme for the
resolution of drugs and their intermediates. The immobilization of ABL on various matrices for its enantioselectivity,stability, and reusability has been studied. Immobilization by covalent bonding on sepharose and silica afforded a maximum of 380 and 40 IU/g activity, respectively,whereas sol 13gel entrapment provided a maximum of 150 IU/g activity in dry powder. The immobilized enzyme
displayed excellent stability in the pH range of 4 1310 and
even at higher temperature, i.e., 50 1360
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/218/1/10.1007s00253-006-0520-5.pdf
Chaubey, Asha and Parshad, Rajinder and Koul, Surrinder and Taneja, Subhash C. and Qazi, Ghulam N. (2006) Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity. Applied Microbiology and Biotechnology, 73 (3). pp. 598-606. ISSN 0175-7598
 
Relation http://dx.doi.org/10.1007/s00253-006-0520-5
http://iiim.csircentral.net/218/