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Arthrobacter sp. lipase immobilization on magnetic sol 13gel composite supports for enantioselectivity improvement

IR@CSIR-IIIM

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Title Arthrobacter sp. lipase immobilization on magnetic sol 13gel composite supports for enantioselectivity improvement
 
Creator Chaubey, Asha
Parshad, Rajinder
Taneja, Subhash C.
Qazi, Ghulam N.
 
Subject Biological Sciences
 
Description A bacterial lipase from Arthrobacter sp. (ABL: IIIM Jammu, India strain, MTCC No. 5125) has been immobilized on non-magnetic (Type A) and magnetic (Type B) supports derived from copolymerization of 3-aminopropyltriethoxysilane and tetraethylorthosilicate.Immobilized ABLpresented 21 1334 mg/g protein binding providing 30 1375 units/g activity in Type A non-magnetic composites and 24 1345 mg/g protein
binding providing 35 1390 units/g activity with Type B supports containing magnetic particles. Immobilized
ABL preparations have shownenhanced stability at pH5 139 and temperature up to 70 8C whereas free ABL is
unstable under these conditions. Improved hydrolytic conversion as well as enantioselectivity were
observedwith acylfluoxetine intermediate (ethyl3-hydroxy-3-phenylpropanoate alkyl acylates) andchiral
auxiliaryacyl 1-phenyl ethanol usingimmobilized ABL derivatives (ee�99%; 3 134-fold increase in E-values)
as compared to ABL enzyme/cells (ee 93 1398%). Introduction of magnetic particles in these supports has led
to easier separation process with high product recovery yields.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/220/1/10.1016j.procbio.2008.10.001.pdf
Chaubey, Asha and Parshad, Rajinder and Taneja, Subhash C. and Qazi, Ghulam N. (2009) Arthrobacter sp. lipase immobilization on magnetic sol 13gel composite supports for enantioselectivity improvement. Process Biochemistry, 44 (2). pp. 154-160. ISSN 13595113
 
Relation http://dx.doi.org/10.1016/j.procbio.2008.10.001
http://iiim.csircentral.net/220/