Record Details

5S rDNA gene diversity in tea (Camellia sinensis (L.) O. Kuntze) and its use for variety identification

IR@CSIR-IHBT

View Archive Info
 
 
Field Value
 
Title 5S rDNA gene diversity in tea (Camellia sinensis
(L.) O. Kuntze) and its use for variety identification
 
Creator Singh, D
Ahuja, Paramvir Singh
 
Subject Plant Biotechnology
 
Description Variability in the organization of repeats of 5S rDNA is useful for phylogenetic studies in various crops. We
found variable repeats of 5S rDNA gene in the genome of tea (Camellia sinensis (L.) O. Kuntze) during Southern hybridization.
Variability in the repeats of 5S rDNA with specific restriction endonuleases (Sau3AI, BamHI, and ApoI)
was analyzed in 28 different tea clones representing 3 types of tea. Our results clearly show that the 5S rDNA gene in
tea could be used as a molecular marker to distinguish C. sinensis Chinary tea from the other important types of tea,
namely Assamica and Cambod. Upon analysis with restriction endonucleases, the 5S rDNA gene in the tea genome
was found to be heavily methylated.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/115/1/2006_5S_rNDA_gene.pdf
Singh, D and Ahuja, Paramvir Singh (2006) 5S rDNA gene diversity in tea (Camellia sinensis (L.) O. Kuntze) and its use for variety identification. Genome, 49. pp. 91-96.
 
Relation http://ihbt.csircentral.net/115/