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A Validated and Densitometric HPTLC Method for the Quantification of Withaferin-A and Withanolide-A in Different Plant Parts of Two Morphotypes of Withania somnifera

IR@CSIR-IHBT

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Title A Validated and Densitometric HPTLC Method
for the Quantification of Withaferin-A
and Withanolide-A in Different Plant Parts
of Two Morphotypes of Withania somnifera
 
Creator Sharma, V
Gupta , A P
Bhandari, P
Gupta , R C
Singh , Bikram
 
Subject Plant sciences
 
Description A simple, sensitive and accurate high performance thin layer chromatographic (HPTLC) method
has been developed for the estimation of withaferin-A and withanolide-A in different plant parts
such as, leaf, root, stem and fruit of two morphotypes of Withania somnifera. HPTLC of W.
somnifera methanolic extract was performed on Si 60 F254 (20 cm · 20 cm) plates with toluene:
ethyl acetate:formic acid (5:5:1), as mobile phase. Quantitative evaluation of the plate was
performed in the absorption-reflection mode at 530 nm. The method was validated for precision,
repeatability, and accuracy. The average recovery of withaferin-A and withanolide-A in
two levels were 96.0 and 96.7%, showing the excellent reproducibility of the method. The
calibration curves were linear for both in the range of 200–3,200 ng. The technique has been
applied, for the first time, for the estimation of withaferin-A and withanolide-A in different parts
of the two morphotypes of Withania somnifera. The method is simple, precise, specific, sensitive
and accurate and can be used for routine analysis as well as for quality control of raw materials
and herbal formulations.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/123/1/2007_A_Valadated.pdf
Sharma, V and Gupta , A P and Bhandari, P and Gupta , R C and Singh , Bikram (2007) A Validated and Densitometric HPTLC Method for the Quantification of Withaferin-A and Withanolide-A in Different Plant Parts of Two Morphotypes of Withania somnifera. Chromatographia, 66. pp. 801-814.
 
Relation http://ihbt.csircentral.net/123/