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Isolation and Characterization of Exopolysaccharides Produced by the Cyanobacterium Limnothrix redekei PUPCCC 116

IR@CSIR-IHBT

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Title Isolation and Characterization of Exopolysaccharides
Produced by the Cyanobacterium Limnothrix redekei
PUPCCC 116
 
Creator Khattar, J I S
Singh, D P
Jindal, N
Kaur, N
Singh, Y
Rahi, P
Gulati, Arvind
 
Subject Microbiology
 
Description Limnothrix redekei PUPCCC 116, a filamentous cyanobacterium, has been
identified through 16S rRNA gene sequencing. Exopolysaccharides (EPS) of this organism
have been isolated and characterized chemically, and its rheological properties were compared
with commercial xanthan. The organism produced 304 μg EPS/ml culture in 21 days. The rate
of EPS production was maximum (313 μg EPS/mg protein/day) during the initial days of
growth, and it decreased to 140 μg EPS/mg protein/day during 18-21 days of growth.
Chemical analysis of EPS revealed the presence of glucose/mannose, ribose, rhamnose, and
uronic acid. Fourier transformed infrared spectrum of EPS further revealed the presence of
methyl and carboxyl groups besides C–N groups indicating the presence of peptidyl moieties.
Elemental analysis of EPS showed the presence of 4.97% N. The organism under continuous
light produced 102% more EPS compared to when grown under a light/dark cycle of 14/10 h.
The rhelological properties of EPS were comparable with commercial xanthan gum.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/135/1/2010_Isolation.pdf
Khattar, J I S and Singh, D P and Jindal, N and Kaur, N and Singh, Y and Rahi, P and Gulati, Arvind (2010) Isolation and Characterization of Exopolysaccharides Produced by the Cyanobacterium Limnothrix redekei PUPCCC 116. Applied Biochemistry and Biotechnology, 162. pp. 1327-1338.
 
Relation http://ihbt.csircentral.net/135/