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Complete nucleotide sequence of cherry virus A (CVA) infecting sweet cherry in India

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Title Complete nucleotide sequence of cherry virus A (CVA) infecting
sweet cherry in India
 
Creator Noorani, M S
Awasthi, P
Singh , R M
Raja, Ram
Sharma, M P
Singh, S R
Hallan, Vipin
Ahmed, N
Zaidi, A A
 
Subject Plant viruses
 
Description Cherry virus A (CVA) is a graft-transmissible
member of the genus Capillovirus that infects different
stone fruits. Sweet cherry (Prunus avium L; family Rosaceae)
is an important deciduous temperate fruit crop in the
Western Himalayan region of India. In order to determine
the health status of cherry plantations and the incidence of
the virus in India, cherry orchards in the states of Jammu
and Kashmir (J&K) and Himachal Pradesh (H.P.) were
surveyed during the months of May and September 2009.
The incidence of CVA was found to be 28 and 13% from
J&K and H.P., respectively, by RT-PCR. In order to
characterize the virus at the molecular level, the complete
genome was amplified by RT-PCR using specific primers.
The amplicon of about 7.4 kb was sequenced and was
found to be 7,379 bp long, with sequence specificity to
CVA. The genome organization was similar to that of
isolates characterized earlier, coding for two ORFs, in
which ORF 2 is nested in ORF1. The complete sequence
was 81 and 84% similar to that of the type isolate at the
nucleotide and amino acid level, respectively, with 50 and
30 UTRs of 54 and 299 nucleotides, respectively. This is the
first report of the complete nucleotide sequence of cherry
virus A infecting sweet cherry in India.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/151/1/2010_Complete.pdf
Noorani, M S and Awasthi, P and Singh , R M and Raja, Ram and Sharma, M P and Singh, S R and Hallan, Vipin and Ahmed, N and Zaidi, A A (2010) Complete nucleotide sequence of cherry virus A (CVA) infecting sweet cherry in India. Archives of Virology, 155 (12). pp. 2079-2082.
 
Relation http://ihbt.csircentral.net/151/