ICAR Research Data Repository for Knowledge Management
Enzymatic resolution of naproxen
IR@CSIR-IIIM
View Archive Info| Field | Value | |
| Title |
Enzymatic resolution of naproxen
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| Creator |
Koul, Surrinder
Parshad, Rajinder Taneja, Subhash C. Qazi, Ghulam N. |
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| Subject |
Chemical Sciences
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| Description |
Trichosporon sp. (TSL), a newly found strain isolated from a locally fermented cottage cheese has been found to be highly stereoselective in the resolution of(S)-(+)-naproxen (ee >99%, E�500) from the corresponding racemic methyl ester. The process of resolution using whole cells has been scaled up to multi-kg level. Optimization of experimental conditions including downstream processing at 80–100 g/L substrate concentration with >90% recovery has been achieved. Changes in the physical parameters such as the particle size of the substrate play an important role in the resolution kinetics. A new strain of Trichosporon sp. having high cell density in cultivation (>60 g dry cell mass L−1 in 14–16 h) is found to be sufficiently stable for two years in dry powder form at 5–8°C. The viability of the resolution process has been further improved by the development of a simple racemization process for the enriched (R)-(−)-ester. |
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| Date |
2003
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/251/1/10.1016S0957-4166%2803%2900492-0.pdf
Koul, Surrinder and Parshad, Rajinder and Taneja, Subhash C. and Qazi, Ghulam N. (2003) Enzymatic resolution of naproxen. Tetrahedron: Asymmetry, 14 (16). pp. 2459-2465. ISSN 09574166 |
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| Relation |
http://dx.doi.org/10.1016/S0957-4166(03)00492-0
http://iiim.csircentral.net/251/ |
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