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Analysis of the Coat Protein Gene of Indian Strain of Apple Stem Grooving Virus

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Title Analysis of the Coat Protein Gene of Indian Strain of Apple Stem
Grooving Virus
 
Creator Negi, A
Rana, T
Kumar, Y
Hallan, Vipin
Raja, Ram
Zaidi, A A
 
Subject Plant sciences
Plant Biotechnology
 
Description A survey was undertaken in the temperate fruit growing regions of Himachal Pradesh (HP) and Jammu & Kashmir (J&K).
Apple stem grooving virus (ASGV), a Capillovirus, was detected in different cultivars of apple, nectarines, plum, cherry,
quince and apricot by double antibody sandwich ELISA (DAS-ELISA). The coat protein (CP) gene sequence of an amplicon
produced by RT-PCR, confirmed the association of ASGV in apple cultivar Starkrimson, collected from Himachal Pradesh.
The CP of Indian ASGV isolate shared 100 % sequence identity with a Brazilian isolate (AF438409). Sequence analysis by
Recombination Detection Program (RDP2) indicated no recombination event for the Indian isolate. However, recombination
was detected in Chinese, Korean and Citrus tatter leaf virus-Taiwan (CTLV) strains of ASGV. The study describes first report
of ASGV infection in India and characterization of its CP gene.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/156/1/13-A-Negi%5B1%5D.pdf
Negi, A and Rana, T and Kumar, Y and Hallan, Vipin and Raja, Ram and Zaidi, A A (2010) Analysis of the Coat Protein Gene of Indian Strain of Apple Stem Grooving Virus. Journal of Plant Biochemistry and Biotechnology, 19 (1). pp. 91-94.
 
Relation http://ihbt.csircentral.net/156/