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Molecular evidence for Apple chlorotic leaf spot virus in wild and cultivated apricot in Himachal Pradesh, India.

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Title Molecular evidence for Apple chlorotic leaf spot virus in wild and cultivated apricot in Himachal Pradesh, India.
 
Creator Rana, T
Chandel, V
Hallan, Vipin
Zaidi, A A
 
Subject Plant viruses
 
Description Wild apricot (Prunus armeniaca) is known as “chuli” or
“zardalu” in Himachal Pradesh (HP, India) where it grows
in the hills at altitudes of 1000-1700 meters. The fruits have
medicinal properties and seedlings are used as rootstocks
for commercial apricot, peach and plum orchards (Parmar
and Kaushal, 1982). Apple chlorotic leaf spot virus
(ACLSV), genus Trichovirus, has been reported to cause
“viruela” and “butteratura” diseases in apricot and is cause
of apricot decline (Sutic et al., 1999). Infections are normally
symptomless but can result in severe graft incompatibility
with some Prunus combinations. During surveys for
ACLSV infection in P. armeniaca, leaf samples (30 from
cultivated and 23 from wild plants) were collected and subjected
to ELISA in triplicate. Positive results were obtained
from 14 samples. Inoculation of leaf extracts of the positive
samples to Chenopodium amaranticolor resulted in small
chlorotic spots on inoculated leaves. RT/PCR using degenerate
primers (Accession Nos. AM490253 and AM490254)
designed to yield amplicons corresponding to the complete
coat protein gene gave a 758 bp product. Sequence analysis
of amplicons from wild (AM498048) and cultivated
(AM498045) apricot virus isolates showed 94% and 95%
identity at the nucleotide and amino acid level among
themselves, and were 92% to 96% identical at the amino
acid level with two apple ACLSV isolates from HP. Indian
ACLSV isolates were similar to others with the exception
of a Turkish isolate [70% similarity (Ulubas and Ertunc,
2005)] (AJ586636). ACLSV from cultivated apricot was
91% similar to an Italian (AJ586635) and a Spanish
(AJ586632) virus isolate. This is the first report of ACLSV
infection of apricot in India, and of ACLSV infection of
wild apricot.
 
Publisher Edizioni ETS , Piazza Carrara 16-19, 56126 Pisa, Italy .
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/170/1/apple.pdf
Rana, T and Chandel, V and Hallan, Vipin and Zaidi, A A (2007) Molecular evidence for Apple chlorotic leaf spot virus in wild and cultivated apricot in Himachal Pradesh, India. Journal of Plant Pathology, 89 (3). p. 572. ISSN 1125-4653
 
Relation http://ihbt.csircentral.net/170/