Record Details

In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia

IR@CSIR-IIIM

View Archive Info
 
 
Field Value
 
Title In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia
 
Creator Ali, I.
Sharma, P.
Suri, K. A.
Satti, N. K.
Dutt, P.
Afrin, F.
Khan, I. A.
 
Subject Biological Sciences
 
Description This study was undertaken to investigate the synergistic interaction between amphotericin B(AmB) and acteoside, isolated from the aerial parts of the shrub Colebrookea oppositifolia(Lamiaceae). Acteoside alone exhibited no intrinsic antifungal activity but showed a potent
synergism in combination with AmB against selected pathogenic species, with fractional inhibitory concentration indices in the range of 0.0312–0.1562. The combination of acteoside at 3.12 and 12.5 mg ml–1 with subinhibitory concentrations of AmB resulted in a potent fungicidal effect and also exhibited a significantly extended post-antifungal effect. Furthermore, the combination also reduced the minimum biofilm reduction concentration values of AmB (2–16-fold) in preformed
biofilms of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. There was decreased viability of the cells, increased uptake of propidium iodide and enhanced leakage of 260 nm-absorbing material by Candida albicans cells when exposed to AmB in the presence of acteoside. The reason for potentiation is likely to be that the subinhibitory concentrations of AmB facilitated the uptake of acteoside, which resulted in increased killing of the fungal cells.Administration of acteoside in mice at up to 2000 mg (kg body weight)”1 by the intraperitoneal or
oral route produced no overt toxicity. The data presented here support synergism between acteoside and AmB, and it is therefore proposed that a prospective new management strategy for therapeutic application of this combination should be explored.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/270/1/10.1099jmm.0.031906-0.pdf
Ali, I. and Sharma, P. and Suri, K. A. and Satti, N. K. and Dutt, P. and Afrin, F. and Khan, I. A. (2011) In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia. Journal of Medical Microbiology, 60 (9). pp. 1326-1336. ISSN 0022-2615
 
Relation http://dx.doi.org/10.1099/jmm.0.031906-0
http://iiim.csircentral.net/270/